The Study on the Relationship between Leaf Colors and ptDNA Inheritance in Intersectional Cross of Rhododendron kiusianum×R. japonicum f. flavum, Resulting in an Unexpected Triploid Progeny
The Study on the Relationship between Leaf Colors and ptDNA Inheritance in Intersectional Cross of Rhododendron kiusianum×R. japonicum f. flavum, Resulting in an Unexpected Triploid Progeny
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黄杜鹃交叉杂交中叶色与ptDNA遗传关系的研究，产生意外的三倍体后代。
DOI：
10.2503/jjshs.71.214
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发表时间：
2002
影响因子：
--
通讯作者：
I. Miyajima
中科院分区：
文献类型：
--
作者：
K. Ureshino;I. Miyajima
2个杂交组合（2n=2x=26） 45株幼苗叶片颜色与质体/核基因组构建的关系×R。以日本黄花（japonicum f. flavum, 2n=2x=26）为研究对象，分析了杜鹃花品种杂交中白化后代频繁出现的原因。幼苗的叶色分成绿色、淡绿色和白化，比例约为1:2:7。通过同工酶分析和染色体观察，在杂种中发现了一株分别具有2倍和1倍核基因组的三倍体绿色植株。PCR-RFLP分析表明，所有白化杂交种的质体DNA （ptDNA）均遗传自金缕金，而7个绿色杂交种中有6个遗传自黄缕金。绿色的三倍体后代具有桃金娘的ptDNA。
The relationship between leaf color and plastid/nuclear genome construction of 45 seedlings from two crosses of R. kiusianum (2n=2x=26)×R. japonicum f. flavum (2n=2x=26) was examined to resolve the frequent appearances of albino progenies among crosses involving Rhododendron species. Leaf color of seedlings segregated into green, pale green, and albino at a ratio, ca. 1 : 2 : 7. An unexpected triploid green plant with 2x and 1x nuclear genome from R. kiusianum and R. japonicum f. flavum, respectively, was detected among the hybrids through isozyme analysis and chromosome observation. PCR-RFLP analysis of matK region revealed that plastid DNA (ptDNA) of all albino hybrids was inherited from R. kiusianum, whereas that of six of seven green hybrids inherited it from R. japonicum f. flavum. The triploid green progeny had ptDNA of R. kiusianum.