Artificial Activation of Murine Oocytes Using Strontium to Derive Haploid and Diploid Parthenotes.
Artificial Activation of Murine Oocytes Using Strontium to Derive Haploid and Diploid Parthenotes.
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DOI：
10.1007/978-1-0716-1979-7_2
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发表时间：
2022-01-01
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通讯作者：
Kalthur, Guruprasad
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作者：
Crasta, Daphne Norma;Adiga, Satish Kumar;Kalthur, Guruprasad
孤雌生殖是低等动物中常见的生殖策略，它涉及卵母细胞发育成胚胎，而没有精子的任何贡献。这种现象在胎盘哺乳动物中自然不会发生。然而，哺乳动物卵母细胞可以在体外使用机械、电和化学刺激人工激活，其可以发育至胚泡阶段。在本章中，我们描述了在体外条件下使用锶作为激活剂从小鼠卵母细胞产生单倍体和二倍体单性生殖体的协议。
Parthenogenesis is a common reproductive strategy among lower animals that involves the development of an embryo from an oocyte, without any contribution from spermatozoon. This phenomenon does not occur naturally in placental mammals. However, the mammalian oocytes can be artificially activated in vitro using mechanical, electrical, and chemical stimuli which can develop up to the blastocyst stage. In this chapter, we describe the protocol for generating haploid and diploid parthenotes from mouse oocytes using strontium as the activating agent under in vitro conditions.