A global profile of replicative polymerase usage.
A global profile of replicative polymerase usage.
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DOI：
10.1038/nsmb.2962
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发表时间：
2015-03
影响因子：
16.8
通讯作者：
Carr AM
中科院分区：
文献类型：
--
作者：
Daigaku Y;Keszthelyi A;Müller CA;Miyabe I;Brooks T;Retkute R;Hubank M;Nieduszynski CA;Carr AM
三种真核DNA聚合酶是基因组复制所必需的。Polα引发酶启动每个合成事件，并迅速被进行性DNA聚合酶取代：Polε复制前导链，而Polδ执行滞后链合成。然而，目前尚不清楚这种分工是否在整个基因组中保持，或者在单个复制子中是否均匀。利用S. pombe，我们已经开发了一种聚合酶使用测序（Pu-seq）策略，以映射聚合酶使用全基因组。Pu-seq提供了直接的复制起点位置和效率数据以及复制时间的间接估计。我们证实，劳动分工是广泛保持在整个基因组。然而，我们的数据表明，在使用的两个聚合酶在个别复制子的微妙的变化。我们认为这是由于Polδ偶尔引发前导链，然后交换为Polε所致。
Three eukaryotic DNA polymerases are essential for genome replication. Polα-primase initiates each synthesis event and is rapidly replaced by processive DNA polymerases: Polε replicates the leading strand while Polδ performs lagging strand synthesis. However, it is not known whether this division of labour is maintained across the whole genome or how uniform it is within single replicons. Using S. pombe, we have developed a polymerase usage sequencing (Pu-seq) strategy to map polymerase usage genome–wide. Pu–seq provides direct replication origin location and efficiency data and indirect estimates of replication timing. We confirm that the division of labour is broadly maintained across an entire genome. However, our data suggest a subtle variability in the usage of the two polymerases within individual replicons. We propose this results from occasional leading strand initiation by Polδ followed by exchange for Polε.