Long-term in vivo electromechanical reshaping for auricular reconstruction in the New Zealand white rabbit model.
Long-term in vivo electromechanical reshaping for auricular reconstruction in the New Zealand white rabbit model.
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DOI：
10.1002/lary.25237
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发表时间：
2015-09
期刊：
影响因子：
--
通讯作者：
Wong BJ
中科院分区：
文献类型：
--
作者：
Badran KW;Manuel CT;Loy AC;Conderman C;Yau YY;Lin J;Tjoa T;Su E;Protsenko D;Wong BJ
旨在证明机电重塑 (EMR) 对体内长期动物模型中软骨形状变化、结构完整性、细胞活力和移植物重塑的剂量学影响。动物研究。在 31 只新西兰白兔的耳廓基部内制造了软骨膜下软骨缺损。自体肋软骨移植物经过机电重塑，类似于兔子耳廓基底框架，并机械固定到耳廓基底缺损中。 49 个肋软骨样本（4 个对照样本和 45 个实验样本）使用一对电压-时间组合成功进行了 EMR，并存活了 6 周或 12 周。测量形状变化，并使用数字成像、组织组织学和共聚焦显微镜以及活-死活力测定对样本进行分析。在所有实验样本中，形状变化与电荷转移成正比（P <.01），并且随着电压的增加而增加。所有实验标本的轮廓都与耳廓基部一致。插入针电极处观察到局灶性软骨变性和纤维化，范围为2.2至3.9毫米。对损伤的反应随着电荷转移和生存时间的增加而增加。 EMR 导致软骨移植物发生适当的形状变化，软骨细胞损伤高度局部化。这些研究表明，使用 EMR 进行耳廓重建可能是可行的。为了评估 EMR 的长期影响和形状变化潜力，有必要延长生存期并进一步优化电压-时间对。
To demonstrate the dosimetry effect of electromechanical reshaping (EMR) on cartilage shape change, structural integrity, cellular viability, and remodeling of grafts in an in vivo long-term animal model. Animal study. A subperichondrial cartilaginous defect was created within the base of the pinna of 31 New Zealand white rabbits. Autologous costal cartilage grafts were electromechanically reshaped to resemble the rabbit auricular base framework and mechanically secured into the pinna base defect. Forty-nine costal cartilage specimens (four control and 45 experimental) successfully underwent EMR using a paired set of voltage-time combinations and survived for 6 or 12 weeks. Shape change was measured, and specimens were analyzed using digital imaging, tissue histology, and confocal microscopy with LIVE-DEAD viability assays. Shape change was proportional to charge transfer in all experimental specimens (P <.01) and increased with voltage. All experimental specimens contoured to the auricular base. Focal cartilage degeneration and fibrosis was observed where needle electrodes were inserted, ranging from 2.2 to 3.9 mm. The response to injury increased with increasing charge transfer and survival duration. EMR results in appropriate shape change in cartilage grafts with chondrocyte injury highly localized. These studies suggest that elements of auricular reconstruction may be feasible using EMR. Extended survival periods and further optimization of voltage-time pairs are necessary to evaluate the long-term effects and shape-change potential of EMR.