ISOLATION OF A DEAD-FAMILY PROTEIN GENE THAT ENCODES A MURINE HOMOLOG OF DROSOPHILA-VASA AND ITS SPECIFIC EXPRESSION IN GERM-CELL LINEAGE
ISOLATION OF A DEAD-FAMILY PROTEIN GENE THAT ENCODES A MURINE HOMOLOG OF DROSOPHILA-VASA AND ITS SPECIFIC EXPRESSION IN GERM-CELL LINEAGE
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DOI：
10.1073/pnas.91.25.12258
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发表时间：
1994-12-06
影响因子：
11.1
通讯作者：
NOCE, T
中科院分区：
文献类型：
--
作者：
FUJIWARA, Y;KOMIYA, T;NOCE, T
为了研究哺乳动物生殖细胞谱系决定过程的分子基础，我们试图分离出与VasA同源的小鼠基因，VasA是果蝇生殖细胞前体形成的母系决定因子。利用家族特异性引物对小鼠原始生殖细胞cDNAs进行逆转录-聚合酶链式反应，获得了一个编码死亡家族蛋白的基因(MVH)，该基因与果蝇VasA基因产物的相似性比以往报道的小鼠基因要高得多。在成体组织中，MVH转录本仅在睾丸生殖细胞中检测到，其中MVH蛋白定位于精母细胞和圆形精子细胞的胞浆中，包括一个核周颗粒。该蛋白也在定植于胚胎性腺的生殖细胞中表达，但在干细胞和生殖细胞等多潜能胚胎细胞中未检测到。这些结果表明，MVH蛋白可能在小鼠生殖细胞的决定事件中发挥重要作用，就像在果蝇中一样。
In an effort to study the molecular basis of the determination processes of the mammalian germ cell lineage, we have tried to isolate a mouse gene homolog to vasa, which plays an essential role as a maternal determining factor for the formation of Drosophila germ cell precursors. By reverse transcriptase PCRs of mouse primordial germ cell cDNAs using family-specific primers, we obtained a gene (Mvh) encoding a DEAD-family protein that showed a much higher degree of similarity with the product of the Drosophila vasa gene (vas) than previously reported mouse genes. In adult tissues, Mvh transcripts were exclusively detected in testicular germ cells, in which Mvh protein was found to be localized in cytoplasm of spermatocytes and round spermatids including a perinuclear granule. The protein was also expressed in germ cells colonized in embryonic gonads but was not detected in pluripotential embryonic cells such as stem cells and germ cells. These results suggest the possibility that the Mvh protein may play an important role in the determination events of mouse germ cells as in the case of Drosophila vasa.