Effect of the Phase Transition in Liposomes on the Fluorescence of Amphiphilic Cyanine Dyes
Effect of the Phase Transition in Liposomes on the Fluorescence of Amphiphilic Cyanine Dyes
复制标题
脂质体相变对两亲性花青染料荧光的影响
DOI：
10.1246/bcsj.53.1914
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发表时间：
1980
影响因子：
4
通讯作者：
M. Sukigara
中科院分区：
文献类型：
--
作者：
K. Onuki;K. Kurihara;Y. Toyoshima;M. Sukigara
研究了两亲性花青染料的荧光强度对脂质双层膜的热致液-固相变的依赖性。将 N,N'-二十八烷基氧杂碳花青碘化物或 N,N'-二十八烷基硫代碳花青溴化物掺入单层脂质体中，观察到每种染料的荧光强度因脂质双层的相变而发生显着改变。具有单长烷基链的两亲性花青染料N-十八烷基-N'-甲基硫代羰花青溴化物也被掺入脂质双层中，但在这种情况下，相变对荧光强度几乎没有影响。这些结果根据双层膜流动性和刚性对掺入的花青染料的旋转运动的影响进行解释。利用这些两亲性染料作为脂质体相变的荧光探针，获得了相变参数。
The dependence of fluorescence intensity of amphiphilic cyanine dyes on the thermotropic fluid-solid phase transition of lipid bilayer membranes has been investigated. N,N′-dioctadecyl oxacarbocyanine iodide or N,N′-dioctadecyl thiacarbocyanine bromide was incorporated into single lamellar liposomes, and the fluorescence intensity of each dye was observed to be modified considerably by the phase transition of the lipid bilayers. An amphiphilic cyanine dye with single long alkyl chain, N-octadecyl-N′-methyl thiacarbocyanine bromide was also incorporated in the lipid bilayers, but in this case, the phase transition showed little effect on the fluorescence intensity. These results were interpreted in terms of the effect of the bilayer membrane fluidity and rigidity on the rotational motion of the incorporated cyanine dyes. The phase transition parameters were obtained by applying those amphiphilic dyes as the fluorescence probe of the phase transition of the liposomes.