Impaired cyclic nucleotide-dependent vasorelaxation in human umbilical artery smooth muscle.
Impaired cyclic nucleotide-dependent vasorelaxation in human umbilical artery smooth muscle.
复制标题
人脐动脉平滑肌中环核苷酸依赖性血管舒张功能受损。
DOI：
10.1152/ajpheart.1995.268.1.h202
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Rasmussen,H
中科院分区：
文献类型：
--
作者：
Bergh,CM;Brophy,CM;Dransfield,DT;Lincoln,T;Goldenring,JR;Rasmussen,H
用毛喉素激活腺苷酸环化酶途径或用硝普钠激活鸟苷酸环化酶途径都不能诱导血清素预收缩的人脐动脉平滑肌（HUASM）的主动松弛，但会导致血清素预收缩的牛颈动脉平滑肌（BCASM）的主动松弛。这种反应差异似乎是 HUASM 所独有的，因为所有其他血管肌肉对这些物质都会表现出松弛。毛喉素和硝普钠刺激导致 HUASM 和 BCASM 中细胞内腺苷 3',5'-环单磷酸 (cAMP) 和鸟苷 3',5'-环单磷酸 (cGMP) 浓度分别增加。 cAMP 和 cGMP 依赖性蛋白激酶均存在于 HUASM 中，并且可以通过分别添加外源 cAMP 和 cGMP 在 HUASM 匀浆中激活。当毛喉素或硝普钠在 BCASM 中起作用时，两种低分子量蛋白质的磷酸化程度会增加。当 HUASM 用任何一种药物处理时，这两种蛋白质都没有显示出这样的增加。因此，HUASM 无法表现出主动松弛似乎是环核苷酸依赖性蛋白激酶活化受损的继发因素。对主动放松的抵抗可能会导致分娩时脐血管系统中发生的血管痉挛。
Activation of either the adenylate cyclase pathway with forskolin or the guanylate cyclase pathway with sodium nitroprusside fails to induce active relaxation of serotonin-precontracted human umbilical artery smooth muscle (HUASM) but causes active relaxation of serotonin-precontracted bovine carotid artery smooth muscle (BCASM). This difference in response appears to be unique to HUASM in that all other vascular muscles exhibit relaxation in response to these substances. Forskolin and sodium nitroprusside stimulation leads to respective increases in intracellular adenosine 3',5'-cyclic monophosphate (cAMP) and guanosine 3',5'-cyclic monophosphate (cGMP) concentrations in HUASM and BCASM. cAMP- and cGMP-dependent protein kinases are both present in HUASM and can be activated in homogenates of HUASM by the addition of exogenous cAMP and cGMP, respectively. When either forskolin or nitroprusside acts in BCASM, two low-molecular-weight proteins display an increase in the extent of phosphorylation. Neither protein shows such an increase when HUASM is treated with either agent. Thus the inability of HUASM to display active relaxation appears to be secondary to impaired activation of cyclic nucleotide-dependent protein kinases. The refractoriness to active relaxation may contribute to the vasospasm that occurs in the umbilical vasculature with parturition.