TLC-electrostatic field induced spray ionization-MS analysis of diverse structural skeletons and its coupling with TLC bioautography for characterization of lipase inhibitory components in American ginseng
TLC-electrostatic field induced spray ionization-MS analysis of diverse structural skeletons and its coupling with TLC bioautography for characterization of lipase inhibitory components in American ginseng
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不同结构骨架的薄层色谱-静电场诱导喷雾电离-质谱分析及其与薄层色谱生物自显影的耦合用于西洋参中脂肪酶抑制成分的表征
DOI：
10.1016/j.jpba.2019.06.019
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发表时间：
2019
影响因子：
3.4
通讯作者：
Cheng Zhihong
中科院分区：
文献类型：
--
作者：
Zhang Peiliang;Zhang Lei;Shi Jiyao;Zhang Na;Li Yue;Wu Tao;Cheng Zhihong
具有不同骨架结构的天然化合物具有不同的电离效率和不同的质谱反应。对影响静电场诱导喷雾电离(EFISI)-MS分析多种天然化合物的关键因素进行了优化和改进。选取生物碱、黄酮类、酚酸类、木脂素类、香豆素类、蒽醌类、单萜类、倍半萜类、二萜类和三萜类等10种常见天然产物的15种参比物质，采用点印迹法研究其在薄层色谱板上的efis - ms响应。获得了这些化合物在正离子模式下的最佳电离条件，以及它们的检测限。此外，为了避免某些化合物在正离子模式下难以电离的局限性，首次开发并优化了TLC-EFISI-MS方法的负离子模式。结合薄层色谱（TLC）生物自显像法，对西洋参中9种脂肪酶抑制成分的efisi - msns数据进行了初步鉴定，并与参比物的irrf值和msns数据进行了比较。这些抑制脂肪酶的化合物有24(S)-伪人参皂苷F11、人参皂苷Rg1、Re、XVII、Rc、Rb2/Rb3、Rb1、rod和丙二酰人参皂苷Rb1。这是首次报道tlc - efisi - ms方法适用于草药结构骨架的广谱分析。
Natural compounds with diverse structural skeletons have different ionization efficiencies and different mass spectrometry (MS) responses. Some key factors influencing the electrostatic field induced spray ionization (EFISI)-MS for a variety of natural compounds have been optimized and improved. Fifteen reference substances representing ten well-known skeletons of natural products including alkaloids, flavonoids, phenolic acids, lignans, coumarins, anthraquinones, monoterpenoids, sesquiterpenoids, diterpenoids and triterpenoids, were selected and investigated for their EFISI-MSnresponses on TLC plates using a dot-blot test. The optimized ionization conditions for these compounds in the positive ion mode were achieved, together with their limits of detection. In addition, to avoid the limitation of some compounds being difficultly ionized in the positive ion mode, the negative ion mode of the TLC-EFISI-MS method was developed and optimized for the first time. By coupling with a TLC bioautographic assay, nine lipase inhibitory components in American ginseng (Panax quinquefoliumroots) have been successfully identified/ tentatively identifiedin situby their EFISI-MSndata and further confirmed by comparisons of theirRfvalues and MSndata with those of reference substances. These lipase inhibitory compounds were 24(S)-pseudo-ginsenoside F11, ginsenosides Rg1, Re, XVII, Rc, Rb2/Rb3, Rb1, Roand malonyl-ginsenoside Rb1. This is the first report that the TLC-EFISI-MSnmethod is adapted to a broad-spectrum analysis of structural skeletons present in herbal medicines.