COMPLEMENTARY EXPRESSION OF PARVALBUMIN AND CALBINDIN D-28K DELINEATES SUBDIVISIONS OF THE RABBIT MEDIAL GENICULATE-BODY
COMPLEMENTARY EXPRESSION OF PARVALBUMIN AND CALBINDIN D-28K DELINEATES SUBDIVISIONS OF THE RABBIT MEDIAL GENICULATE-BODY
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DOI：
10.1002/cne.903590407
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发表时间：
1995-09-04
影响因子：
2.5
通讯作者：
MCMULLEN, NT
中科院分区：
文献类型：
--
作者：
DEVENECIA, RK;SMELSER, CB;MCMULLEN, NT
用钙结合蛋白小白蛋白(PV)和钙结合蛋白D-28K(CB)免疫组织化学互补染色方法，对兔内侧膝状体(MGB)的四个主要亚区进行了定位。PV免疫反应主要见于腹侧和内侧部，而CB免疫反应细胞主要见于背侧部和内侧部。腹侧核由于密集的神经纤维标记和中等标记的胞体而呈强烈的PV+。内侧核既有中等大小的PV+胞体，也有粗大的PV+轴突和终末。楔形内核由密集标记的CB+细胞组成，在吻侧分离背侧核和腹侧核，并向尾部扩张，包裹MGV后部。大的多极CB+神经元与放射状树突是背核的特征。PV和CB的不同表达也区分了背侧部的深背侧亚核和背侧浅亚核，以及腹侧部的腹外侧成分。与不同物种中MGB连接的研究相比较表明，从下丘中央核接受大量输入并投射到初级听觉皮质的亚群中，PV免疫反应性最高。相反，CB免疫反应的特点是主要从其他来源接受输入的核，如下丘、外侧被盖和脊髓的中央旁核，并投射到次级听觉区域。钙结合蛋白免疫组织化学能够跨越模糊的细胞结构边界描绘神经元的隔间，使其成为指导未来对MGB的连接和生理研究的有力工具。(C)1995年Wiley-Liss公司
The complementary pattern of immunohistochemical staining for the calcium-binding proteins parvalbumin (PV) and calbindin D-28k (CB) was used to delineate four major subdivisions of the rabbit medial geniculate body (MGB). PV immunoreactivity predominates in the ventral and medial divisions, whereas CB-immunoreactive cells characterize the dorsal and internal divisions. The ventral nucleus is strongly PV+ due to dense neuropil labeling and moderately labeled somata. The medial nucleus contains both medium-sized and large PV+ somata, as well as thick PV+ axons and terminals. The wedge-shaped internal nucleus, composed of densely labeled CB+ cells, separates the dorsal and ventral nuclei rostrally, and expands caudally to encapsulate the posterior MGV. Large multipolar CB+ neurons with radiate dendrites characterize the dorsal nucleus. The differential expression of PV and CB also distinguishes the deep dorsal and superficial dorsal subnuclei in the dorsal division and a ventrolateral component in the ventral division. A comparison with studies of MGB connectivity in a variety of species suggests that PV immunoreactivity is highest in subdivisions that receive a substantial input from the central nucleus of the inferior colliculus and that project to primary auditory cortex. In contrast, CB immunoreactivity characterizes nuclei that receive input primarily from other sources, such as the paracentral nuclei of the inferior colliculus, the lateral tegmentum, and the spinal cord, and that project to secondary auditory areas. The ability of calcium-binding protein immunohistochemistry to delineate neuronal compartments across indistinct cytoarchitectonic borders makes it a powerful tool for guiding future connectional and physiological studies of the MGB. (C) 1995 Wiley-Liss, Inc.