Effect of biohydrogen by overexpressing small RNA RyhB combined with ldh impairment in novel Klebsiella sp. FSoil 024
Effect of biohydrogen by overexpressing small RNA RyhB combined with ldh impairment in novel Klebsiella sp. FSoil 024
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DOI：
10.1016/j.ijhydene.2021.03.087
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发表时间：
2021-04
影响因子：
7.2
通讯作者：
Wan-Ru Chu;Yudong Xu;Jiayao Yang;Wu Yan;Xuan Wei;Hao Yaqiao;Shenghou Wang;Hongxin Zhao
中科院分区：
文献类型：
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作者：
Wan-Ru Chu;Yudong Xu;Jiayao Yang;Wu Yan;Xuan Wei;Hao Yaqiao;Shenghou Wang;Hongxin Zhao
生物技术制氢被认为是石油化学源或电解法的一种环境可持续替代方案。在这里，通过阻断乳酸脱氢酶(LDH)和通过小RNA RyhB调节代谢来提高Klebsiellasp的产氢能力。菌株FSoil 024。在5-L发酵罐中，FSoil024-L(ΔLDH)和FSoil024-L/R(ΔLDH/RyhB)的葡萄糖产氢量分别比野生型提高了40%和50%。当采用甘油作为较有利的底物时，FSoil 024-L发酵52小时后产生3.3%L/L的氢气，表明其利用粗甘油制氢的潜力很大。RyhB的过表达下调了FSoil 024中甲酸盐的生物合成，从而将NADH重新定向到产氢途径。这一发现为细胞还原能力在氢代谢中的作用提供了新的见解，并为提高氢气产量奠定了理论基础。
Biotechnological hydrogen production is considered as an environmentally sustainable alternative to petrochemical sources or electrolysis. Here, disruption of lactate dehydrogenase (LDH) and metabolic regulation via the small RNA RyhB were adopted to improve hydrogen production in the novelKlebsiellasp. strain FSoil 024. The hydrogen production of FSoil 024-L (Δldh) and FSoil 024-L/R (Δldh/RyhB) from glucose in a 5-L fermenter respectively increased by 40 and 50% compared to the wild type. When glycerol was adopted as a more favorable substrate, FSoil 024-L generated 3.3 L/L of hydrogen after 52 h of fermentation, implying its great potential for the utilization of crude glycerol to produce hydrogen. Overexpression of RyhB downregulated formate biosynthesis in FSoil 024, thereby redirecting NADH toward the hydrogen production pathway. This finding provides new insights into the role of cellular reducing power in hydrogen metabolism and establishes a rationale for improving hydrogen production.