Altered expression of the septin gene, SEPT9, in ovarian neoplasia
Altered expression of the septin gene, SEPT9, in ovarian neoplasia
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DOI：
10.1002/path.1484
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发表时间：
2003-12-01
影响因子：
7.3
通讯作者：
Russell, SHE
中科院分区：
文献类型：
--
作者：
Burrows, JF;Chanduloy, S;Russell, SHE
隔蛋白基因家族参与了多种细胞过程，包括胞质分裂、膜转运和融合、胞吐和凋亡。Septin家族的一个成员映射到染色体17q25.3，这是一个在散发性卵巢和乳腺肿瘤中通常缺失的区域，并且也被鉴定为急性髓性白血病中MLL的融合伴侣。目前的研究表明，这种septin基因的多个剪接变体的表达模式在卵巢肿瘤和细胞系中发生改变。特别是，zeta转录本的表达在大多数肿瘤和细胞系中是可检测的，但在一系列非恶性成人和胎儿组织中是不可检测的。Zeta表达伴随着普遍存在的β转录物的丢失。在卵巢肿瘤中未检测到该基因的体细胞突变，但已证明肿瘤细胞系中的β表达可通过5-氮杂胞苷治疗重新激活，表明甲基化在控制该基因表达中的作用。版权所有（C）2003约翰威利父子有限公司。
The septin family of genes has been implicated in a variety of cellular processes including cytokinesis, membrane transport and fusion, exocytosis, and apoptosis. One member of the septin family maps to chromosome 17q25.3, a region commonly deleted in sporadic ovarian and breast tumours, and has also been identified as a fusion partner of MLL in acute myeloid leukaemias. The present study demonstrates that the pattern of expression of multiple splice variants of this septin gene is altered in ovarian tumours and cell lines. In particular, expression of the zeta transcript is detectable in the majority of tumours and cell lines, but not in a range of non-malignant adult and fetal tissues. Zeta expression is accompanied by loss of the ubiquitous beta transcript. Somatic mutations of the gene were not detected in ovarian tumours, but it was demonstrated that beta expression in tumour cell lines can be reactivated by 5-azacytidine treatment, suggesting a role for methylation in the control of expression of this gene. Copyright (C) 2003 John Wiley Sons, Ltd.