RELATIONSHIP BETWEEN IGG1 AND IGG4 ANTIBODIES TO FOODS AND THE DEVELOPMENT OF IGE ANTIBODIES TO INHALANT ALLERGENS .1. ESTABLISHMENT OF A SCORING SYSTEM FOR THE OVERALL FOOD RESPONSIVENESS AND ITS APPLICATION TO 213 UNSELECTED CHILDREN
RELATIONSHIP BETWEEN IGG1 AND IGG4 ANTIBODIES TO FOODS AND THE DEVELOPMENT OF IGE ANTIBODIES TO INHALANT ALLERGENS .1. ESTABLISHMENT OF A SCORING SYSTEM FOR THE OVERALL FOOD RESPONSIVENESS AND ITS APPLICATION TO 213 UNSELECTED CHILDREN
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DOI：
10.1111/j.1365-2222.1991.tb00809.x
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发表时间：
1991-01-01
影响因子：
6.1
通讯作者：
AALBERSE, RC
中科院分区：
文献类型：
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作者：
CALKHOVEN, PG;AALBERS, M;AALBERSE, RC
为了获得后续调查的参考水平，我们分析了213名儿童（年龄3个月至14岁）血清中常见食物的IgG 1和IgG 4抗体水平。 这些儿童被分成五个年龄组，并在一个广泛的普通食物筛选小组中进行测试。 我们使用IgG 1和IgG 4 RAST与琼脂糖偶联抗原：牛奶、鸡蛋白色、香蕉、豆类（大豆和花生的混合物）、谷物（小麦和大米的混合物）、马铃薯、橙子和猪肉。 在所有年龄组和所有抗原中，抗体反应存在相当大的变异性，对于某些检测，超过一半的血清为阴性或临界值，基于区间或顺序标度的统计被认为是不合适的，我们采用名义分类。 我们决定，对于每项测定，使用年龄组的75百分位数作为临界水平。 因此，每个抗体滴度被转换为阳性（超过该年龄组的75百分位数）或阴性;阳性检测的数量被用作评分。 这产生了SIGMA-G1-评分和SIGMA-G4评分（分别为IgG 1和IgG 4抗体的总分）。 本研究的结果表明，对一种食物有高反应的儿童往往对其他非相关食物有更高的反应，这可能是由粘膜屏障缺陷和/或免疫系统过度活跃所解释的。 这表明可能存在高食物反应表型。
To obtain reference levels for subsequent investigations, we analysed the IgG1 and IgG4 antibody levels to common foods in the sera of 213 unselected children (age 3 months to 14 years). The children were clustered into five age groups and tested on a broad screening panel of common foods. We used the IgG1 and IgG4 RAST with Sepharose-coupled antigens: cows' milk, hens' egg white, banana, legumes (a mixture of soybean and peanut), grains (a mixture of wheat and rice), potato, orange and pork. In all age groups and all antigens, a considerable variability in the antibody response was found. As for some assays more than half of the sera were negative or borderline, statistics based on interval or ordinal scaling were considered inappropriate and we resorted to nominal classification. We decided to use, for each of the assays, the 75-percentile of the age group as a cut-off level. Each antibody titre was thus converted into positive (more than the 75-percentile of that age group) or negative; the number of positive tests was used as the score. This resulted in a SIGMA-G1-SCORE AND A SIGMA-G4 score (summed scores for IgG1 and IgG4 antibodies, respectively). The results of the present study indicate that children with a high response to one food tend to have elevated responses to other non-related foods, possibly explained by a defective mucosal barrier and/or a hyperactive immune system. This suggests that a high-food responder phenotype may exist.