Antioxidant properties of various solvent extracts of total phenolic constituents from three different agroclimatic origins of drumstick tree (Moringa oleifera Lam.) leaves
Antioxidant properties of various solvent extracts of total phenolic constituents from three different agroclimatic origins of drumstick tree (Moringa oleifera Lam.) leaves
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DOI：
10.1021/jf020444
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发表时间：
2003-04-09
影响因子：
6.1
通讯作者：
Becker, K
中科院分区：
文献类型：
--
作者：
Siddhuraju, P;Becker, K
辣木冻干叶的水、水甲醇和水乙醇提取物。研究了来自不同农业气候区的自由基清除能力和抗氧化活性。所有叶提取物均具有清除过氧自由基和超氧自由基的能力。不同溶剂提取物对稳定的1,1-二苯基- 2-吡啶肼基（DPPH,）自由基的清除活性相似。在3种不同的辣木样品中，印度产的甲醇和乙醇提取物在-胡萝卜素-亚油酸体系中的抗氧化活性最高，分别为65.1和66.8%。但随着各提取物浓度的增加，其还原能力均显著提高（P < 0.05），这可能是其抗氧化活性的部分原因。酚类化合物的主要生物活性成分为类黄酮，如槲皮素和山奈酚。根据所获得的结果，辣木叶因其显著的抗氧化活性而被发现是天然抗氧化剂的潜在来源。本文首次报道了辣木叶冻干提取物的抗氧化性能。总体而言，甲醇（80%）和乙醇（70%）是提取辣木叶中抗氧化化合物的最佳溶剂。
Water, aqueous methanol, and aqueous ethanol extracts of freeze-dried leaves of Moringa oleifera Lam. from different agroclimatic regions were examined for radical scavenging capacities and antioxidant activities. All leaf extracts were capable of scavenging peroxyl and superoxyl radicals. Similar scavenging activities for different solvent extracts of each collection were found for the stable 1,1-diphenyl 2-picrylhydrazyl (DPPH,) radical. Among the three different moringa samples, both methanol and ethanol extracts of Indian origins showed the highest antioxidant activities, 65.1 and 66.8%, respectively, in the beta-carotene-linoleic acid system. Nonetheless, increasing concentration of all the extracts had significantly (P < 0.05) increased reducing power, which may in part be responsible for their antioxidant activity. The major bioactive compounds of phenolics were found to be flavonoid groups such as quercetin and kaempferol. On the basis of the results obtained, moringa leaves are found to be a potential source of natural antioxidants due to their marked antioxidant activity. This is the first report on the antioxidant properties of the extracts from freeze-dried moringa leaves. Overall, both methanol (80%) and ethanol (70%) were found to be the best solvents for the extraction of antioxidant compounds from moringa leaves.