Generation of targeted retroviral vectors by using single-chain variable fragment: an approach to in vivo gene delivery.
Generation of targeted retroviral vectors by using single-chain variable fragment: an approach to in vivo gene delivery.
复制标题
使用单链可变片段生成靶向逆转录病毒载体：一种体内基因递送方法。
DOI：
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发表时间：
1995
影响因子：
11.1
通讯作者：
Inder M. Verma
中科院分区：
文献类型：
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作者：
N. Somia;Monica Zoppè;Inder M. Verma
我们报道了一种逆转录病毒载体的产生，该载体通过识别低密度脂蛋白受体特异性感染人类细胞。这种靶向感染的基本原理是在莫洛尼鼠白血病病毒的亲嗜包膜蛋白（通常对鼠细胞具有营养性）上添加来自识别人类低密度脂蛋白受体的单克隆抗体的单链可变片段。该嵌合包膜蛋白用于构建产生逆转录病毒载体的包装细胞系，该逆转录病毒载体能够将大肠杆菌β-半乳糖苷酶基因高效转移至表达低密度脂蛋白受体的人类细胞。这种方法提供了生成细胞和组织特异性逆转录病毒载体的通用计划，这是体内基因治疗策略的重要一步。
We report the generation of a retroviral vector that infects human cells specifically through recognition of the low density lipoprotein receptor. The rationale for this targeted infection is to add onto the ecotropic envelope protein of Moloney murine leukemia virus, normally trophic for murine cells, a single-chain variable fragment derived from a monoclonal antibody recognizing the human low density lipoprotein receptor. This chimeric envelope protein was used to construct a packaging cell line producing a retroviral vector capable of high-efficiency transfer of the Escherichia coli beta-galactosidase gene to human cells expressing low density lipoprotein receptor. This approach offers a generalized plan to generate cell and tissue-specific retroviral vectors, an essential step toward in vivo gene therapy strategies.