Early histological and ultrastructural changes in expanded murine scalp
Early histological and ultrastructural changes in expanded murine scalp
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扩大的小鼠头皮的早期组织学和超微结构变化
DOI：
10.1080/01913123.2020.1720876
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发表时间：
2020-01-28
影响因子：
1
通讯作者：
Ma, Xianjie
中科院分区：
文献类型：
--
作者：
Yu, Zhou;Liu, Shiqiang;Ma, Xianjie
摘要组织扩张已广泛用于整形、重建和美容手术。小鼠头皮扩张模型可以有效地模仿人类皮肤扩张的特征。然而，对扩张头皮的组织学特征和超微结构特征，特别是早期超微结构变化缺乏详细的研究。在此，建立小鼠头皮扩张模型，并在最终注射后第2天（组I）和第4天（组II）获得扩张的头皮样品。组织学分析显示，表皮增厚，真皮变薄，皮下脂肪变薄，并在扩大的样品囊形成。超微结构评价显示存在角质形成细胞，具有许多张力原纤维和受损的线粒体，并且在真皮和囊中观察到几根断裂的胶原纤维和增加数量的活性成纤维细胞和肌成纤维细胞。在两组的扩增样品中均检测到脂肪细胞去分化，但仅在I组的真皮成纤维细胞中检测到自噬体的形成。因此，应仔细监测扩张组织的早期变化，因为这可能有助于避免皮肤变薄并促进扩张组织再生。
ABSTRACT Tissue expansion has been widely used for plastic, reconstructive, and esthetic surgeries. A mouse scalp expansion model can effectively mimic the characteristics of human skin expansion. However, a detailed study of the histological features and ultrastructural characteristics of expanded scalp is lacking, especially early ultrastructural changes. Here, a mouse scalp expansion model was established and the expanded scalp samples were obtained on day 2 (group I) and 4 (group II) post final injection. Histological analysis revealed epidermal thickening, dermal thinning, subcutaneous fat thinning, and capsule formation in the expanded samples. Ultrastructural evaluation showed the presence of keratinocytes with numerous tonofibrils and damaged mitochondria, and several ruptured collagen fibers and increased number of active fibroblasts and myofibroblasts were observed in the dermis and capsules. Adipocyte dedifferentiation was detected in the expanded samples of both groups, but formation of autophagosomes was only detected in the dermal fibroblasts of group I. Thus, early changes in expanded tissue should be carefully monitored, as it may help avoid dermal thinning and promote expanded tissue regeneration.