Depletion of cells of the B lineage in the bone marrow of zinc-deficient mice.
Depletion of cells of the B lineage in the bone marrow of zinc-deficient mice.
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发表时间：
1995-05
期刊：
影响因子：
6.4
通讯作者：
L. King;F. Osati-Ashtiani;P. Fraker
中科院分区：
文献类型：
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作者：
L. King;F. Osati-Ashtiani;P. Fraker
虽然淋巴细胞减少症经常在营养不良的人类和啮齿动物中发现，但对次优营养对淋巴细胞生成过程的影响知之甚少。主要关注年轻成年小鼠骨髓中的B谱系细胞，中度缺锌（MZD）导致携带B220的有核细胞比例下降43%，在更严重缺锌小鼠（SZD）中观察到91%的下降。早期B细胞（B220+IG-）对这种缺陷高度敏感，在SZD小鼠中几乎检测不到，在MZD小鼠中几乎减少了60%。未成熟的B细胞（B220+IgM+IgD-）也受到类似影响，根据缺乏程度下降35%至80%。在MZD小鼠中，成熟B细胞（IgM+IgD+）表现出中度损失，具有一定的抗性。在SZD小鼠中观察到该群体中更严重的损失。流式细胞仪（FACS）的散点图表明，锌缺乏引起的比例急剧下降的小有核细胞，在骨髓中被认为含有高比例的发展中的淋巴样细胞。伴随着大颗粒细胞的增加，MZD和SZD小鼠携带Mac-1的有核细胞比例大幅增加。由于缺锌造成骨髓中B谱系细胞的急剧耗竭，骨髓中淋巴细胞生成过程的破坏可能在许多类型的营养不良中观察到的淋巴细胞减少症中起关键作用。
Though lymphopenia is often noted in malnourished humans and rodents, little is known about the effects of suboptimal nutriture on lymphopoietic processes. Focusing primarily on cells of the B lineage in the marrow of young adult mice, a moderate degree of zinc deficiency (MZD) caused a 43% decline in the proportion of nucleated cells bearing B220 with a 91% decline noted among more severely zinc deficient mice (SZD). Early B cells (B220+Ig-) were highly sensitive to the deficiency, being barely detectable in SZD mice and reduced by almost 60% in MZD mice. Immature B cells (B220+IgM+IgD-) were similarly affected, declining 35% to 80% depending on the degree of the deficiency. In MZD mice, mature B cells (IgM+IgD+) exhibited moderate losses, being somewhat resistant. A more profound loss in this population was noted for SZD mice. Flow cytometric (FACS) scatter profiles indicated that zinc deficiency caused a sharp decline in the proportion of small nucleated cells which in the marrow are thought to contain a high proportion of developing lymphoid cells. There was a concomitant increase in large granular cells that paralleled a substantial increase in the proportion of nucleated cells bearing Mac-1 for both MZD and SZD mice. Given the dramatic depletion of cells of the B lineage in the marrow created by a deficiency in zinc, it is probable that disruptions in lymphopoietic processes in the marrow play a key role in the resulting lymphopenia observed in many types of malnutrition.