Genotype and interleukin-10 responses after cardiopulmonary bypass
Genotype and interleukin-10 responses after cardiopulmonary bypass
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DOI：
10.1093/bja/aeg174
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发表时间：
2003-09-01
影响因子：
9.8
通讯作者：
Webster, NR
中科院分区：
文献类型：
--
作者：
Galley, HF;Lowe, PR;Webster, NR
背景。促炎和抗炎细胞因子的平衡与炎症状况的结果有关，而体外循环与明显的炎症反应有关。白细胞介素 10 (IL-10) 是一种抗炎细胞因子，在创伤或手术后发生败血症的患者中其水平最高。 IL-10 水平因个体而异，基因型可能决定 IL-10 反应。因此，我们研究了 IL-10 基因型、循环 IL-10 浓度以及体外循环后 24 小时器官功能障碍的结果。方法。从体外循环前、体外循环后 3 小时和 24 小时采集 150 名患者的血样。 IL-10通过酶免疫测定法测定。通过限制性片段长度多态性分析检测-1082个碱基对的单核苷酸多态性。前瞻性地定义了搭桥后器官系统功能障碍。结果。旁路术后 3 小时 IL-10 浓度增加（P
Background. The pro- and anti-inflammatory cytokine balance has been implicated in outcome from inflammatory conditions, and cardiopulmonary bypass is associated with a marked inflammatory response. Interleukin-10 (IL-10) is an anti-inflammatory cytokine and levels have been shown to be highest in those patients who develop sepsis after trauma or surgery. IL-10 levels vary between individuals and genotype may dictate the IL-10 response. We therefore investigated IL-10 genotype, circulating IL-10 concentrations and outcome in terms of organ dysfunction 24 h after cardiopulmonary bypass.Methods. Blood samples were obtained from 150 patients before, and 3, and 24 h after cardiopulmonary bypass. IL-10 was measured by enzyme immunoassay. The single nucleotide polymorphism at -1082 base pairs was detected by restriction fragment length polymorphism analysis. Post-bypass organ system dysfunction was defined prospectively.Results. IL-10 concentrations were increased 3 h after bypass (P