γ-glutamyl transpeptidase expression in Ewing's sarcoma cells:: up-regulation by interferons
γ-glutamyl transpeptidase expression in Ewing's sarcoma cells:: up-regulation by interferons
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DOI：
10.1042/bj20011854
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发表时间：
2002-06-15
影响因子：
4.1
通讯作者：
Bauvois, B
中科院分区：
文献类型：
--
作者：
Bouman, L;Sancéau, J;Bauvois, B
The genetic hallmark of Ewing's sarcoma family of tumours (ET) is the presence or the translocation t(11;22)(q24;q12). which creates the ET fusion gene, leading to cellular transformation. Five human gamma-glutamyl transpeptidase (gamma-GT) genes are located near the chromosomal translocation in ET, gamma-GT is a major enzyme involved in glutathione homoeostasis. Five human cell lines representative of primary or metastatic tumours were investigated to study whether gamma-GT alterations could occur at the chromosomal breaks and rearrangements in ET. As shown by enzymic assays and FACS analyses, all ET cell lines consistently expressed a functional gamma-GT which however did not discriminate steps of ET progression, As shown previously [Sanceau, Hiscott, Delattre and Wietzerbin (2000) Oncogene 19, 3372-3383], ET cells respond to the antiproliferative effects of interferons (IFNs) type I (alpha and beta) and to a much less degree to IFN type II (gamma). IFN-alpha and -beta arrested cells in the S-phase of the cell cycle. We found an enhancement of gamma-GT mRNA species with IFN-alpha and -beta by reverse transcriptase-PCR analyses. This is reflected by up-regulation of gamma-GT protein, which coincides with the increase in gamma-GT-specific enzymic activity. Similarly, IFNs up-regulate the levels of gamma-GT in another IFN-responsive B cell line. Whether this Lip-regulation of gamma-GT by IFNs is of physiological relevance to cell behaviour remains to be studied.
