Calcium-regulatory mechanisms. Functional classification using skinned fibers.
Calcium-regulatory mechanisms. Functional classification using skinned fibers.
复制标题
钙调节机制。
DOI：
10.1085/jgp.77.2.177
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Malencik,DA
中科院分区：
文献类型：
--
作者：
Kerrick,WG;Hoar,PE;Cassidy,PS;Bolles,L;Malencik,DA
本研究的主要目的是确定是否可以使用各种试剂（3-硫代三磷酸腺苷 [ATP gamma S]、三氟拉嗪 [TFP]、肌钙蛋白 I、环腺苷 3',5'-单磷酸依赖性蛋白激酶 [C 亚基] 的催化亚基和钙调蛋白 [CaM]）对带皮纤维类型进行分类， 然后确定所提出的Ca2+调节机制是否与结果一致。预期改变轻链激酶磷酸酶系统的药物（ATP γ S、TFP、C 亚基、CaM）会强烈影响带皮砂囊平滑肌纤维中 Ca2+ 激活的张力，而这些药物对带皮哺乳动物横纹肌纤维和扇贝内收肌纤维没有影响。已知肌钙蛋白 I 与肌钙蛋白 C 和 CaM 强烈结合，可抑制带皮哺乳动物横纹纤维和砂囊纤维的 Ca2+ 激活，但不会抑制扇贝内收肌的 Ca2+ 激活。不同类型的皮纤维的结果与所提出的 Ca2+ 调节机制一致。
The primary purpose of this study was to determine whether various agents (adenosine 3-thiotriphosphate [ATP gamma S], trifluoperazine [TFP], troponin I, the catalytic subunit of the cyclic adenosine 3',5'-monophosphate dependent protein kinase [C-subunit], and calmodulin [CaM]) could be used to classify skinned fiber types, and then to determine whether the proposed mechanisms for Ca2+ regulation were consistent with the results. Agents (ATP gamma S, TFP, C-subunit, CaM) expected to alter a light chain kinase-phosphatase system strongly affect the Ca2+-activated tension in skinned gizzard smooth muscle fibers, whereas these agents have no effect on skinned mammalian striated and scallop adductor fibers. Troponin I, which is known to bind strongly to troponin C and CaM, inhibits Ca2+ activation of skinned mammalian striated and gizzard fibers but not scallop adductor muscle. The results in different types of skinned fibers are consistent with proposed mechanisms for Ca2+ regulation.