Immunofluorescent analysis of plasma fibronectin incorporation into the lung during acute inflammatory vascular injury.
Immunofluorescent analysis of plasma fibronectin incorporation into the lung during acute inflammatory vascular injury.
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急性炎症性血管损伤期间血浆纤连蛋白掺入肺部的免疫荧光分析。
DOI：
10.1164/ajrccm/148.2.467
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Guinta,C
中科院分区：
文献类型：
--
作者：
Charash,WE;Vincent,PA;Saba,TM;Minnear,FL;McKeown-Longo,PJ;Migliozzi,JA;Lewis,MA;Lewis,E;Guinta,C
成年公羊(25~35 kg)手术前禁食48h。全麻诱导用2.5%硫喷妥钠(15 mg/kg：雅培实验室，芝加哥，伊利诺伊州)。动物经气管插管，机械通气(哈佛仪器，马萨诸塞州米尔斯)，呼吸速度为18次/分钟，潮气量为12ml/kg。绵羊吸入77.5%~78%N20、21%02‘和1%~1.5%氟烷。肺淋巴瘘和股前淋巴瘘的手术准备是在相同的手术间隔(1~1.5h)内无菌条件下进行的，如前所述(14)。手术入路是通过第七肋间的右胸骨切开。对于肺瘘，结扎纵隔尾侧淋巴结的后部，以减少横隔淋巴管对肺淋巴的污染。用聚乙烯(PE-240)导管无菌插管右侧颈总动脉，导管内注入2%十三烷基甲基氯化铵-(TDMAC)-肝素复合体。颈外静脉用8.5号法国Cordis导入器(佛罗里达州迈阿密Cordis实验室)无菌插管。导管沿颈部右侧外化。伤口用0号丝绸缝合。绵羊在一个特殊的术后代谢恢复室康复，可以免费获得食物和水。
Adult male sheep (25 to 35 kg) were fasted of solid food for 48 h before surgery. General anesthesia was induced with 2.5% thiopental sodium (15 mg/kg: Abbott Laboratories, Chicago, IL). The animals were endotracheally intubated and mechanically ventilated (Harvard Apparatus, Mills, MA) at a rate of 18 breaths/min and a tidal volume of 12 ml/kg. The sheep were maintained on inhalation of 77.5 to 78% N20, 21% 02'and 1 to 1.5% halothane. Surgical preparation of the lung lymph fistula as well as the prefemoral lymph fistula was performed under sterile conditions during the same operative interval (1 to 1.5 h), as described previously (14). The surgical approach was through a right thoracotomy via the seventh intercostal space. For the lung fistula, the posterior portion of the caudal mediastinal lymph node was ligated to minimize contamination of lung lymph by diaphragmatic lymphatics. The right common carotid artery was aseptically cannulated with a polyethylene (PE-240) catheter impregnated with 2% tridodecylmethyl ammonium chloride-(TDMAC)-heparin complex. The external jugular vein was aseptically cannulated with a No. 8.5 French Cordis introducer (Cordis Laboratories, Miami, FL). The cannulas were externalized along the right lateral side of the neck. The incision was closed with No. 0 silk. Sheep recovered in a special postoperative metabolic recovery unit with free access to food and water.