Transcription factor GATA-6 activates expression of gastroprotective trefoil genes TFF1 and TFF2
Transcription factor GATA-6 activates expression of gastroprotective trefoil genes TFF1 and TFF2
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DOI：
10.1016/s0167-4781(00)00013-0
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发表时间：
2000-02-29
期刊：
影响因子：
--
通讯作者：
Gött, P
中科院分区：
文献类型：
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作者：
Al-azzeh, ED;Fegert, P;Gött, P
胃肠道炎症和上皮恢复的早期事件之一是三叶因子家族（TFF）分泌肽的上调，TFF促进细胞迁移，保护和愈合粘膜。它们的主要表达部位为胃（TFF1、TFF2）和肠（TFF3）。GATA转录因子位于基因的5'侧区，是控制肠道特异性基因表达的几个公认位点。通过逆转录- pcr (RT-PCR)， GATA-6在胃、肠和胰腺多种肿瘤细胞系中均有表达。在MKN45、KATOIII和LS174T中，用TFF报告基因和GATA-6表达载体共转染发现，GATA-6激活TFF1和TFF2 4-6倍，对TFF3无影响。利用TFF2缺失构建的报告基因分析和凝胶转移实验进一步表征了反向GATA结合序列的功能贡献。(C) 2000 Elsevier Science B.V.版权所有
One of the early events in inflammation and epithelial restitution of the gastrointestinal tract is the up-regulation of secretory peptides belonging to the trefoil factor family (TFF) that promote cell migration, protect and heal the mucosa. Their major expression site is stomach (TFF1, TFF2) and intestine (TFF3). Located in the 5'-flanking region of the genes are several consensus sites for members of the GATA transcription factors known to control gut-specific gene expression. By reverse transcription-PCR (RT-PCR), GATA-6 was shown to be expressed in a variety of tumor cell lines of gastric, intestinal and pancreatic origin. In MKN45, KATOIII and LS174T, cotransfection with TFF reporter genes and GATA-6 expression vectors revealed that GATA-6 activates TFF1 and TFF2 4-6-fold, without an effect on TFF3. The functional contribution of GATA binding sequences in the reverse orientation was further characterized by reporter gene assays using TFF2 deletion constructs and by gel shift experiments. (C) 2000 Elsevier Science B.V. All rights reserved.