Polyethyleneimine nanoparticles incorporated into resin composite cause cell death and trigger biofilm stress in vivo
Polyethyleneimine nanoparticles incorporated into resin composite cause cell death and trigger biofilm stress in vivo
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DOI：
10.1073/pnas.1010341107
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发表时间：
2010-12-21
影响因子：
11.1
通讯作者：
Weiss, Ervin I.
中科院分区：
文献类型：
--
作者：
Beyth, Nurit;Yudovin-Farber, Ira;Weiss, Ervin I.
在牙科树脂复合材料中掺入交联季铵盐聚乙烯亚胺（QPEI）纳米颗粒具有持久而广泛的抗菌作用，对体外生物相容性无明显影响。我们假设将QPEI纳米颗粒结合到树脂复合材料中在体内具有强大的抗菌作用，并且这种应激条件会触发细菌生物膜中的自杀模块。10名志愿者佩戴了一个可移动的丙烯酸器具，其中插入了两个对照树脂复合标本和两个含有1% wt/wt QPEI纳米颗粒的树脂复合标本，以允许口腔内生物膜的形成。4 h后，取出标本，用共聚焦激光扫描显微镜检测细菌活力和生物膜厚度。经QPEI处理后，生物膜厚度增加（p < 0.05），而活性率降低了50% （p < 0.00001）。体外测试了生物膜上清液恢复细菌死亡的能力。体外试验表明，活菌数量减少70% （p < 0.05）。在扫描电镜下也观察到生物膜形态的差异，树脂复合材料与树脂复合材料纳入QPEI。这些结果强烈表明，低浓度的QPEI纳米颗粒掺入树脂复合材料中具有显著的体内抗生物膜活性，并表现出对唾液细菌的有效广谱抗菌活性。
Incorporation of cross-linked quaternary ammonium polyethylenimine (QPEI) nanoparticles in dental resin composite has a long-lasting and wide antimicrobial effect with no measured impact on biocompatibility in vitro. We hypothesized that QPEI nanoparticles incorporated into a resin composite have a potent antibacterial effect in vivo and that this stress condition triggers a suicide module in the bacterial biofilm. Ten volunteers wore a removable acrylic appliance, in which two control resin composite specimens and two resin composite specimens incorporating 1% wt/wt QPEI nanoparticles were inserted to allow the buildup of intraoral biofilms. After 4 h, the specimens were removed and tested for bacterial vitality and biofilm thickness, using confocal laser scanning microscopy. The vitality rate in specimens incorporating QPEI was reduced by >50% (p < 0.00001), whereas biofilm thickness was increased (p < 0.05). The ability of the biofilm supernatant to restore bacterial death was tested in vitro. The in vitro tests showed a 70% decrease in viable bacteria (p < 0.05). Biofilm morphological differences were also observed in the scanning electron microscope micrographs of the resin composite versus the resin composite incorporating QPEI. These results strongly suggest that QPEI nanoparticles incorporated at a low concentration in resin composite exert a significant in vivo antibiofilm activity and exhibit a potent broad spectrum antibacterial activity against salivary bacteria.