Effects of 3-methylcholanthrene on the transcriptional activity and mRNA accumulation of the oncogene hWAPL
Effects of 3-methylcholanthrene on the transcriptional activity and mRNA accumulation of the oncogene hWAPL
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DOI：
10.1016/j.canlet.2004.08.006
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发表时间：
2005-04-18
期刊：
影响因子：
9.7
通讯作者：
Mukai, K
中科院分区：
文献类型：
--
作者：
Kuroda, M;Oikawa, K;Mukai, K
hWAPL是与子宫颈癌相关的人类癌基因。在这里，我们证明，hWAPL转录诱导3-甲基胆蒽（3-MC）在宫颈癌衍生的细胞系SiHa。通过定量真实的时间PCR分析来分析hWAPL转录，并评估mRNA和异质核RNA（hnRNA）水平。流式细胞术分析表明，hWAPL mRNA水平的变化是独立的细胞周期的。我们还发现DMSO和FBS的某些组分影响hWAPL的转录。有趣的是，当芳香烃受体（AhR）功能被α-萘甲酮（ANF）抑制时，3-MC对hWAPL转录的诱导作用大于AhR功能正常细胞。这些观察结果表明，有复杂的机制调节hWAPL的转录。腹腔注射3-MC可诱导小鼠子宫中hWAPL同源物的mRNA水平。因此，3-MC暴露对子宫的某些影响可能是由hWAPL的非程序性过表达介导的。(c)2004爱思唯尔爱尔兰有限公司保留所有权利。
hWAPL is a human oncogene associated with uterine cervical cancer. Here, we demonstrate that hWAPL transcription is induced by 3-methylcholanthrene (3-MC) in the cervical carcinoma-derived cell line SiHa. hWAPL transcription was analyzed with evaluation of the mRNA and heterogeneous nuclear RNA (hnRNA) levels by quantitative real time PCR analysis. Flow cytometric analysis suggested that the alteration of hWAPL mRNA levels is independent of cell cycle profile. We also found that DMSO and some components of FBS affect hWAPL transcription. Interestingly, when the aryl hydrocarbon receptor (AhR) function was inhibited by alpha-naphthoflavone (ANF), the induction of hWAPL transcription by 3-MC was greater than that in AhR-functioning normal cells. These observations suggest that there are complex mechanisms regulating the transcription of hWAPL. Furthermore, mRNA level of a mouse homolog of hWAPL in mouse uterus was induced by 3-MC injection into the abdominal cavity. Thus, some effects from 3-MC exposure on uterus may be mediated by the unscheduled overexpression of hWAPL. (c) 2004 Elsevier Ireland Ltd. All rights reserved.