Microfluidic library screening for mapping antibody epitopes
Microfluidic library screening for mapping antibody epitopes
复制标题
DOI：
10.1021/ac0616916
复制
发表时间：
2007-03-01
影响因子：
7.4
通讯作者：
Daugherty, Patrick S.
中科院分区：
文献类型：
--
作者：
Bessette, Paul H.;Hu, Xiaoyuan;Daugherty, Patrick S.
使用一次性微流体设备筛选分子库的能力提供了简化和自动化试剂生成以及开发用于临床诊断的集成生物分析系统的潜力。在这里，使用一次性微流体装置绘制抗体表位，以筛选由显示在细菌细胞上的 5 x 10(8) 成员组成的组合肽库。芯片上文库筛选是在两级连续流微流体分选机中实现的，该分选机通过介电泳漏斗分离微球上捕获的抗体结合靶细胞。鉴定出的抗体指纹与使用最先进的商业细胞分选仪器获得的抗体指纹相当。
The capability to screen molecular libraries using disposable microfluidic devices provides the potential to simplify and automate reagent generation and to develop integrated bioanalytical systems for clinical diagnostics. Here, antibody epitopes were mapped using a disposable microfluidic device to screen a combinatorial peptide library composed of 5 x 10(8) members displayed on bacterial cells. On-chip library screening was achieved in a two-stage, continuous-flow microfluidic sorter that separates antibody-binding target cells captured on microspheres through dielectrophoretic funneling. The antibody fingerprints identified were comparable to those obtained using state-of-the-art commercial cell sorting instrumentation.