The Salmonella genomic island 1 is specifically mobilized in trans by the IncA/C multidrug resistance plasmid family.
The Salmonella genomic island 1 is specifically mobilized in trans by the IncA/C multidrug resistance plasmid family.
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DOI：
10.1371/journal.pone.0015302
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发表时间：
2010-12-20
期刊：
影响因子：
3.7
通讯作者：
Doublet B
中科院分区：
文献类型：
--
作者：
Douard G;Praud K;Cloeckaert A;Doublet B
沙门氏菌基因组岛 1 (SGI1) 是一种源自肠沙门氏菌的整合可移动元件 (IME)，包含在几种肠沙门氏菌血清型和奇异变形杆菌中鉴定出的各种复杂的多重耐药整合子。先前的研究表明，在 IncA/C 接合辅助质粒 pR55 存在的情况下，SGI1 通过反式动员进行水平转移。在这里，我们报道了不同流行的多药耐药（MDR）质粒，包括携带超广谱β-内酰胺酶（ESBL）基因的质粒，动员多药耐药基因组岛SGI1的能力。通过接合实验，IncFI、FII、HI2、I1、L/M、N、P 不相容组的 24 个接合质粒中没有一个能够以可检测的水平动员 SGI1（转移频率 <10−9）。在我们的收集中，携带ESBL基因的质粒主要来自IncHI2和I1组，因此无法动员SGI1。然而，SGI1 的水平转移被证明是由广泛宿主范围 IncA/C 不相容组的接合辅助质粒特异性介导的。几个接合 IncA/C MDR 质粒以及已测序的 143,963 bp 的 IncA/C 参考质粒 pRA1 显示可从肠沙门氏菌供体反式 SGI1 转移至大肠杆菌受体菌株。根据所使用的 IncA/C 质粒，SGI1 的接合转移发生频率范围为每个供体 10−3 至 10−6 个转接合子。特别值得关注的是，一些携带超广谱头孢菌素酶 bla CMY-2 基因的大型 IncA/C MDR 质粒显示出在反式 SGI1 中动员。 IncA/C MDR 质粒家族动员 SGI1 的能力可能有助于其在肠道病原体之间水平转移的传播。此外，全世界耐多药肠沙门氏菌中 IncA/C 质粒的流行率不断增加，这对抗生素抗性基因组岛 SGI1 及其密切衍生物的流行成功具有潜在影响。
The Salmonella genomic island 1 (SGI1) is a Salmonella enterica-derived integrative mobilizable element (IME) containing various complex multiple resistance integrons identified in several S. enterica serovars and in Proteus mirabilis. Previous studies have shown that SGI1 transfers horizontally by in trans mobilization in the presence of the IncA/C conjugative helper plasmid pR55. Here, we report the ability of different prevalent multidrug resistance (MDR) plasmids including extended-spectrum β-lactamase (ESBL) gene-carrying plasmids to mobilize the multidrug resistance genomic island SGI1. Through conjugation experiments, none of the 24 conjugative plasmids tested of the IncFI, FII, HI2, I1, L/M, N, P incompatibility groups were able to mobilize SGI1 at a detectable level (transfer frequency <10−9). In our collection, ESBL gene-carrying plasmids were mainly from the IncHI2 and I1 groups and thus were unable to mobilize SGI1. However, the horizontal transfer of SGI1 was shown to be specifically mediated by conjugative helper plasmids of the broad-host-range IncA/C incompatibility group. Several conjugative IncA/C MDR plasmids as well as the sequenced IncA/C reference plasmid pRA1 of 143,963 bp were shown to mobilize in trans SGI1 from a S. enterica donor to the Escherichia coli recipient strain. Depending on the IncA/C plasmid used, the conjugative transfer of SGI1 occurred at frequencies ranging from 10−3 to 10−6 transconjugants per donor. Of particular concern, some large IncA/C MDR plasmids carrying the extended-spectrum cephalosporinase bla CMY-2 gene were shown to mobilize in trans SGI1. The ability of the IncA/C MDR plasmid family to mobilize SGI1 could contribute to its spread by horizontal transfer among enteric pathogens. Moreover, the increasing prevalence of IncA/C plasmids in MDR S. enterica isolates worldwide has potential implications for the epidemic success of the antibiotic resistance genomic island SGI1 and its close derivatives.
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影响因子：
4.9
作者：
Cloeckaert, Axel;Praud, Karine;Weill, Francois-Xavier
通讯作者：
Weill, Francois-Xavier
影响因子：
3.2
作者：
CHABBERT, YA;BOUANCHAUD, DH;GERBAUD, GR
通讯作者：
GERBAUD, GR
影响因子：
4.4
作者：
Lindsey, Rebecca L.;Fedorka-Cray, Paula J.;Meinersmann, Richard J.
通讯作者：
Meinersmann, Richard J.
DOI：
10.1099/00221287-65-3-343
发表时间：
1971-01-01
期刊：
JOURNAL OF GENERAL MICROBIOLOGY
影响因子：
--
作者：
AOKI, T;EGUSA, S;WATANABE, T
通讯作者：
WATANABE, T
影响因子：
5.2
作者：
McIntosh, Douglas;Cunningham, Michelle;Ritchie, Rachael
通讯作者：
Ritchie, Rachael