2-DE and MS analysis of key proteins in the adhesion of Lactobacillus plantarum, a first step toward early selection of probiotics based on bacterial biomarkers
2-DE and MS analysis of key proteins in the adhesion of Lactobacillus plantarum, a first step toward early selection of probiotics based on bacterial biomarkers
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DOI：
10.1002/elps.200800399
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发表时间：
2009-03-01
期刊：
影响因子：
2.9
通讯作者：
Ennahar, Saied
中科院分区：
文献类型：
--
作者：
Izquierdo, Esther;Horvatovich, Peter;Ennahar, Saied
鉴定参与益生菌活动的细胞成分是当前益生菌研究中的一个挑战。在这项工作中，使用了一种基于蛋白质组学的新方法作为一种分析工具，以识别与粘蛋白黏附相关的特征蛋白质谱作为益生菌特性的模型。对3株不同粘附率的植物乳杆菌进行蛋白质组学分析：植物乳杆菌WHE 92(15.9%)、植物乳杆菌299v(9.1%)和植物乳杆菌CECT 4185(1.4%)。用双向凝胶电泳法、胰酶消化、CHIP-LC-QTOF质量分析和数据库检索进行蛋白质组学鉴定。此前报道的几种与细菌黏附有关的蛋白质：延伸因子EF-Tu、GroEL伴侣蛋白、分子伴侣DNAK和甘油醛-3-磷酸脱氢酶在高黏附菌株L.PLANARUM WHE 92的细胞壁蛋白质组中过表达。在粘附性最强的菌株中，两个含有Groes辅助伴侣蛋白的斑点过表达，也表明该蛋白参与了粘附性过程。蛋白质组图谱和蛋白质与特殊的益生菌特性的关联为使用这种图谱和蛋白质作为细菌特性的细菌生物标记物开辟了道路，而且可能还用于它们潜在的健康影响。
The identification of cell components involved in probiotic activities is a challenge in current probiotic research. In this work, a new approach based on proteomics as an analytical tool for the identification of characteristic protein profiles related to adhesion to mucin as a model probiotic property was used. Three Lactobacillus plantarum strains with different adhesion rates were used for proteomic analysis: L. plantarum WHE 92 (15.9%), L. plantarum 299 v (9.1%) and L. plantarum CECT 4185 (1.4%). Cell wall extracts were subjected to proteomic analysis of differential protein expression using 2-DE, tryptic digestion, chip-LC-QTOF mass analysis and protein identification using database search. Several proteins, previously reported to be involved in bacterial adhesion: elongation factor EF-Tu, GroEL chaperonin, molecular chaperone DnaK and glyceraldehyde-3-phosphate dehydrogenase were found to be overexpressed in the cell wall proteome of the highly adhesive strain L. plantarum WHE 92. The overexpression of two spots containing GroES co-chaperonin in the most adhesive strain also suggested the involvement of this protein in the adhesion process. The association of proteomic profiles and proteins with particular probiotic properties opens the way for the use of such profiles and proteins as bacterial biomarkers for the properties of bacteria but probably also for their potential health effects.