Expression of Ephs and ephrins in developing mouse inner ear
Expression of Ephs and ephrins in developing mouse inner ear
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DOI：
10.1016/s0378-5955(03)00029-7
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发表时间：
2003-04-01
期刊：
影响因子：
2.8
通讯作者：
Pickles, JO
中科院分区：
文献类型：
--
作者：
Pickles, JO
通过半定量逆转录聚合酶链反应测量发育中的小鼠整个内耳和新生小鼠内耳的解剖部分中编码不同 Ephs 和 ephrins 的 mRNA 的表达水平。对 24 种已知的 Ephs 和 ephrins 中的 19 种进行了调查。结果显示，在胚胎年龄 (E) 11.5 天到 E12.5 之间，每单位组织的水平增加了 10-300 倍。在新生小鼠中，含有Corti和螺旋神经节联合器官的部分显示出相对较强的EphA4、EphB3、ephrin-A3、ephrin-B2和ephrin-B3的表达。在侧壁中，EphA4、ephrin-A3和ephrin-B2强烈表达，而ephrin-A3在椭圆囊和囊状感觉上皮中特别强烈表达。结果表明，Ephs 和 ephrins 可能在内耳结构的分化中发挥作用，并表明哪些 Ephs 和 ephrins 最有可能在内耳结构的分化中发挥重要作用。 (C) 2003 Elsevier Science B.V. 保留所有权利。
Levels of expression of mRNAs encoding the different Ephs and ephrins were measured by semi-quantitative reverse-transcription polymerase chain reaction in developing mouse whole inner ears, and in dissected fractions of the neonatal mouse inner ear. Nineteen of the 24 known Ephs and ephrins were surveyed. The results showed that between embryonic age (E) 11.5 days and E12.5, levels increased 10-300 times per unit of tissue. In neonatal mice, the fraction containing combined organ of Corti and spiral ganglion showed relatively strong expression of EphA4, EphB3, ephrin-A3, ephrin-B2 and ephrin-B3. In the lateral wall, EphA4, ephrin-A3 and ephrin-B2 were strongly expressed, while ephrin-A3 was particularly strongly expressed in utricular and saccular sensory epithelia. The results suggest that the Ephs and ephrins are likely to play a part in the differentiation of the structures of the inner ear, and show which Ephs and ephrins are most likely to play important roles in the different structures. (C) 2003 Elsevier Science B.V. All rights reserved.