Tween 80-modified hyaluronic acid-ss-curcumin micelles for targeting glioma: Synthesis, characterization and their in vitro evaluation.
Tween 80-modified hyaluronic acid-ss-curcumin micelles for targeting glioma: Synthesis, characterization and their in vitro evaluation.
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DOI：
10.1016/j.ijbiomac.2018.09.034
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发表时间：
2018-09
影响因子：
8.2
通讯作者：
Cihui Tian;Sajid Asghar;Yurui Xu;Zhipeng Chen;Jingwei Zhang;Q. Ping;Yanyu Xiao
中科院分区：
文献类型：
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作者：
Cihui Tian;Sajid Asghar;Yurui Xu;Zhipeng Chen;Jingwei Zhang;Q. Ping;Yanyu Xiao
基于亲水性透明质酸-β-疏水性姜黄素偶联物的氧化还原敏感胶束被设计为一种新型的胶质瘤靶向给药系统。此外，所获得的胶束进一步用吐温80（CUR-THSC）官能化以获得更好的脑渗透。动态光散射实验和体外释药研究表明，合成的二硫键偶联物在高谷胱甘肽条件下具有氧化还原敏感性。得到了平均粒径为74.2 nm、负zeta电位（-30.25 mV）、高包封率（94.12%）和载药量（8.9%）的球形胶束。胶束的XRD分析显示包封药物的无定形形式。CUR-THSC胶束显示出良好的血浆稳定性，并且在红细胞中不诱导任何溶血。此外，与游离姜黄素组和非敏感胶束组相比，观察到G422细胞的最高细胞毒性。这些结果表明，吐温80修饰的透明质酸-β-姜黄素胶束可以成为一个有前途的平台，用于交付姜黄素治疗胶质瘤。
Redox-sensitive micelles based onhydrophilic hyaluronic acid-ss-hydrophobic curcumin conjugate were designed as a novel delivery system for gliomas targeting. Furthermore, the obtained micelles were further functionalized with Tween 80 (CUR-THSC) for better brain penetration. Dynamic light scattering experiment andin vitrorelease study showed that the synthetic disulfide-linked conjugate possessed redox-sensitivity under high glutathione conditions. Spherical micelles with a mean particle size of 74.2 nm, negative zeta potential (−30.25 mV), high entrapment efficiency (94.12%) and drug loading (8.9%) were obtained. XRD analysis of micelles revealed amorphous form of the encapsulated drug. CUR-THSC micelles showed good plasma stability and did not induce any hemolysis in erythrocytes. In addition, highest cytotoxicity in G422 cells was observed compared to the free curcumin group and non-sensitive micelles group. These results indicate that the Tween 80-modified hyaluronic acid-ss-curcumin micelles could emerge as a promising platform for the delivery of curcumin in the treatment of gliomas.