HISTOCHEMICAL, BIOCHEMICAL, AND CONTRACTILE PROPERTIES OF RED, WHITE, AND INTERMEDIATE FIBERS
HISTOCHEMICAL, BIOCHEMICAL, AND CONTRACTILE PROPERTIES OF RED, WHITE, AND INTERMEDIATE FIBERS
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DOI：
10.1152/ajplegacy.1971.220.2.410
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发表时间：
1971-01-01
影响因子：
--
通讯作者：
PETER, JB
中科院分区：
文献类型：
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作者：
BARNARD, RJ;EDGERTON, VR;PETER, JB
方法选用成年雄性哈特利豚鼠（> ~ 700 g）。选取比目鱼肌、拇长屈肌（FHL）、指长屈肌（FDL）、腓骨内侧肌（MG）和股外侧肌部分粗红（RV）为研究对象。用于组织化学分析的肌肉迅速从动物身上移除，并去除脂肪和结缔组织。每块肌肉的腹部都被切除，放在一个固定的卡盘上，用液氮快速冷冻。然后从每块肌肉上切下连续切片（6,u），放在玻璃罩上染色。nadh -二磷酸腺苷酶（NADH-D）活性测定采用Novikoff等人（14）的方法，肌球蛋白atp酶活性测定采用Padykula和Herman（16）的方法以及对Guth和Samaha（11）方法的改进。从每张幻灯片上拍照并放大，以便对单个纤维进行分类。对50多块比目鱼肌、4块FHL肌、4块FDL肌、10块股外侧肌（红色部分）和13块MG肌的横截面进行了分析。单个纤维被分为红色、白色或中间纤维。中间纤维的特点是肌球蛋白三磷酸腺苷酶的浅色染色和在nadh -二磷酸腺苷酶反应中均匀分散的双双胍颗粒的独特模式。红色和白色纤维都被肌球蛋白三磷酸腺苷酶染色。红色和白色的区分是主观上由NADH-D反应产生的。红色纤维的特征是大量的粗二甲酰胺颗粒，最集中在肌层下区域。白色纤维的特征是很少有双甲酰胺颗粒，在肌层下区域也最突出。这些组织化学特征如图1、A和b所示。关于组织化学技术和纤维分类方法的进一步细节已在其他地方发表(8)。
METHODSAdult (> 700 g) male Hartley guinea pigs were used. The muscles selected for study were the soleus, flexor hallucis longus (FHL), flexor digitorum longus (FDL), medial gastrocnemius(MG), and the portion of the vastus lateralis which is grossly red (RV).Histochemistry. The muscles used for histochemical analysis were rapidly removed from the animals and trimmed free of fat and connective tissue. The belly of each muscle was ablated and placed on a mounting chuck for quick freezing with liquid nitrogen. Serial sections (6, u) were then cut from each muscle and placed on cover glasses for staining. NADH-diaphorase(NADH-D) activity was determined by the method of Novikoff et al.(14) and myosin ATPase activity by the method of Padykula and Herman (16) and a modification of Guth and Samaha (11) method. Photographs were taken from each slide and enlarged to permit classification of individual fibers. Cross sections of the muscles were analyzed from more than 50 soleus muscles, 4 FHL, 4 FDL, 10 vastus lateralis (red portion), and 13 MG. Individual fibers were classified as red, white, or intermediate. Intermediate fibers are characterized by their light staining with myosin ATPase and their distinct pattern of small, uniformly dispersed diformazan granules in the NADH-diaphorase reaction. Both red and white fibers are dark staining with myosin ATPase. The division into red and white is subjectively made from the NADH-D reaction. Red fibers are characterized by an abundance of coarse diformazan granules which are most concentrated in the subsarcolemmal region. White fibers are characterized by few diformazan granules which are also most prominent in the subsarcolemmal region. These histochemical characteristics are shown in Fig. 1, A and B. Further details concerning the histochemical techniques and methods of fiber classification have been published elsewhere (8).