ABP41 is involved in the pollen tube development via fragmenting actin filaments.
ABP41 is involved in the pollen tube development via fragmenting actin filaments.
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DOI：
10.1093/mp/ssn073
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发表时间：
2008-11
期刊：
影响因子：
27.5
通讯作者：
Ting Wang;Y. Xiang;Jian-Yun Hou;H. Ren
中科院分区：
文献类型：
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作者：
Ting Wang;Y. Xiang;Jian-Yun Hou;H. Ren
ABP 41是百合花粉中植物绒毛蛋白/凝溶胶蛋白/片段蛋白超家族的一个新成员，在体外能与肌动蛋白丝化学计量结合并切断肌动蛋白丝。为了进一步了解其在体内的功能和潜在的分子机制，进行了生化分析，荧光显微镜观察和显微注射实验。不同的生化测量表明，ABP 41在体外维持肌动蛋白丝的短F-肌动蛋白的形式。将ABP 41注射到花粉管中，可使已存在的肌动蛋白丝断裂，抑制胞质流动速度，缩短花粉管透明带长度。此外，还发现在花粉萌发的不同时期，内源ABP 41的表达水平与花粉中的短肌动蛋白丝结构动态对应。我们的研究结果表明，ABP 41是参与调节肌动蛋白动态在花粉萌发过程中，通过维护短动态肌动蛋白丝。
ABP41 is identified as a novel member of plant villin/gelsolin/fragmin superfamily proteins from lily pollen, which binds stoichiometrically to actin filaments and severs them in vitro. To further understand its in-vivo function and the potential molecular mechanisms, biochemical analysis, fluorescence microscopic observation and microinjection assays were performed. Different biochemical measurements showed that ABP41 maintained actin filaments in forms of short F-actin in vitro. Microinjection of ABP41 into pollen tubes could fragment the pre-existing actin filaments, inhibit the velocity of cytoplasmic streaming, and shorten the length of the clear zone of pollen tube. In addition, it was found that the endogenous ABP41 expressing level was dynamically corresponding to the short actin filament structure in pollen at different stages of pollen germination. Our results suggest that ABP41 is involved in the regulation of actin dynamics during the pollen germination process via maintenance of short dynamic actin filaments.