Localization of relaxin during formation of the porcine corpus luteum.
Localization of relaxin during formation of the porcine corpus luteum.
复制标题
猪黄体形成过程中松弛素的定位。
DOI：
10.1095/biolreprod40.4.835
复制
发表时间：
1989
影响因子：
3.6
通讯作者：
Ainsworth,L
中科院分区：
文献类型：
--
作者：
Bagnell,CA;Ayau,E;Downey,BR;Tsang,BK;Ainsworth,L
用亲和素-生物素免疫过氧化物酶方法和纯化的松弛素(RLX)抗血清，研究了松弛素(RLX)在猪发情周期黄体组织中的存在和定位。用孕马血清促性腺激素(PMSG)和人绒毛膜促性腺激素(HCG)诱导青春期前母猪排卵。在排卵期和排卵后第19天的特定时间行剖腹手术获取卵巢。重点是每隔12小时和24小时获取卵巢组织，直到排卵后96小时。排卵前和排卵后6h，内膜(TI)细胞均可见RLX免疫染色。排卵后18h，黄体形成的颗粒(G)细胞首次出现与TI细胞相似的RLX免疫染色。随着黄体化的进展，很难确定RLX免疫染色细胞的来源。然而，随着黄体的发育，RLX免疫染色的强度逐渐增强，并定位于大的黄体细胞。到排卵后第19天，RLX免疫染色已检测不到。这些结果表明，RLX在CL的形成和功能寿命过程中存在。此外，排卵后G细胞中RLX的存在似乎与细胞黄体化有关。
The presence and localization of relaxin (RLX) in luteal tissue during the estrous cycle of the pig have been studied using the avidin-biotin immunoperoxidase method and homologous antisera to purified RLX. Prepubertal gilts were induced to ovulate by treatment with pregnant mare’s serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG). Ovaries were obtained at laparotomy during the periovulatory period and at specified times through Day 19 post-ovulation. Emphasis was placed on obtaining ovarian tissue at 12- and 24-h intervals up to 96 h after ovulation. RLX immunostaining was evident in theca interna (TI) cells before and at 6 h after ovulation. At 18 h after ovulation, RLX immunostaining comparable to that seen in TI cells was observed for the first time in luteinizing granulosa (G) cells. As luteinization progressed, it became difficult to identify the origin of the RLX immunostaining cells. However, the intensity of RLX immunostaining increased with corpus luteum (CL) development, with the staining becoming localized in the large luteal cells. By Day 19 after ovulation, RLX immunostaining was undetectable. These results indicate RLX is present in the CL during its formation and functional lifespan. Also, it would appear that the presence of RLX in G cells post-ovulation is associated with cell luteinization.