Glucose induces FGF21 mRNA expression through ChREBP activation in rat hepatocytes
Glucose induces FGF21 mRNA expression through ChREBP activation in rat hepatocytes
复制标题
DOI：
10.1016/j.febslet.2009.07.053
复制
发表时间：
2009-09-03
期刊：
影响因子：
3.5
通讯作者：
Horikawa, Yukio
中科院分区：
文献类型：
--
作者：
Iizuka, Katsumi;Takeda, Jun;Horikawa, Yukio
成纤维细胞生长因子21（FGF 21）具有改善糖尿病啮齿动物血糖和血脂的有益作用。在这里，我们研究了碳水化合物反应元件结合蛋白（ChREBP）参与肝脏中FGF 21 mRNA表达的调节。葡萄糖刺激和腺病毒过表达显性活性ChREBP增加FGF 21 mRNA。因此，显性失活Mlx的腺病毒表达抑制了葡萄糖对FGF 21 mRNA的诱导作用。此外，小鼠FGF 21基因启动子（-2000至+65 bp）的缺失研究揭示了-74至-52 bp之间的葡萄糖响应区域。这些结果表明，FGF 21的表达是由ChREBP调节。(C)2009年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
Fibroblast growth factor 21 (FGF21) has beneficial effects of improving the plasma glucose and lipid profiles in diabetic rodents. Here, we investigated carbohydrate response element binding protein (ChREBP) involvement in the regulation of FGF21 mRNA expression in liver. Glucose stimulation and adenoviral overexpression of dominant active ChREBP increased FGF21 mRNA. Consistently, adenoviral expression of dominant negative Mlx inhibited glucose induction of FGF21 mRNA. Furthermore, deletion studies of mouse FGF21 gene promoter (-2000 to +65 bp) revealed a glucose responsive region between -74 and -52 bp. These findings suggest that FGF21 expression is regulated by ChREBP. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.