Evaluating viral interference between Influenza virus and Newcastle disease virus using real-time reverse transcription-polymerase chain reaction in chicken eggs
Evaluating viral interference between Influenza virus and Newcastle disease virus using real-time reverse transcription-polymerase chain reaction in chicken eggs
复制标题
使用鸡蛋中的实时逆转录聚合酶链反应评估流感病毒和新城疫病毒之间的病毒干扰
DOI：
10.1186/1743-422x-9-128
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发表时间：
2012-07-01
期刊：
影响因子：
4.8
通讯作者：
Wang, Zhiliang
中科院分区：
文献类型：
--
作者：
Ge, Shengqiang;Zheng, Dongxia;Wang, Zhiliang
背景：用无特定病原体(SPF)的鸡蛋同时和顺序接种流感病毒(AIV)和新城疫病毒(NDV)的尿囊腔接种表明，在混合感染过程中，AIV和NDV的相互作用是可变的。本研究通过实时逆转录聚合酶链式反应(Real-time RT-PCR)检测禽流感病毒和新城疫病毒在混合感染中的复制干扰潜力。结果：将不同剂量的新城疫病毒(Lasota或F48E8)和禽流感病毒(F98或H5N1)同时接种到鸡胚蛋中，对新城疫病毒的生长有干扰作用，而对禽流感病毒的生长没有影响。结论：禽流感病毒同时或连续接种对新城疫病毒的生长有负面影响，干扰程度取决于所用毒株的数量和相对毒力，但未观察到对禽流感病毒的干扰。只有早期接种新城疫病毒，新城疫病毒才能干扰禽流感病毒的生长。
Background: Simultaneous and sequential allantoic cavity inoculations of Specific-pathogen-free (SPF) chicken eggs with Influenza virus (AIV) and Newcastle disease virus (NDV) demonstrated that the interaction of AIV and NDV during co-infection was variable. Our research revisited the replication interference potential of AIV and NDV using real-time reverse transcription-polymerase chain reaction (real-time RT-PCR) for AIV and NDV to specifically detect the viral genomes in mixed infections.Results: Data from this survey showed that when different doses of NDV (Lasota or F48E8) and AIV (F98 or H5N1) were simultaneously inoculated into embryonating chicken eggs (ECE), interference with the growth of NDV occurred, while interference with the growth of AIV did not occur. When equal amount of the two viruses were sequentially employed, the degree of interference was dependent upon the time of superinfection and the virulence of virus.Conclusion: AIV have a negative impact on NDV growth if they are inoculated simultaneously or sequentially and that the degree of interference depended upon the quantity and relative virulence of the virus strains used; however, interference with AIV was not observed. Only if NDV were inoculated at an earlier time will NDV able to interfere with the growth of AIV.