Structure of rat atrial natriuretic factor precursor deduced from cDNA sequence.
Structure of rat atrial natriuretic factor precursor deduced from cDNA sequence.
复制标题
从 cDNA 序列推导的大鼠心房钠尿因子前体的结构。
作者：
Maki,M;Takayanagi,R;Misono,KS;Pandey,KN;Tibbetts,C;Inagami,T
心脏的心房含有肽，称为心房利钠因子（ANF），具有利尿1和平滑肌松弛2 - 4活性。鉴于其对肾脏中盐代谢和血管平滑肌的有效作用，ANF被认为在控制液体容量和血管功能中起重要作用。已经分离出几种大小不同的ANF肽3 - 10，并测定了它们的氨基酸序列4，6-10。肽序列的分析表明，它们是通过蛋白水解从相同的前体衍生的。为了验证这一假设，我们已经克隆了cDNA的心钠素前体大鼠心房mRNA，确定其核苷酸序列和推导其氨基酸序列。ANF前体由152个氨基酸残基组成，包括推定的信号肽序列。该序列包含迄今报道的所有ANF肽的氨基酸序列4，6-10。
The atrium of the heart contains peptides, termed atrial natriuretic factors (ANFs), diuretic1and smooth-muscle-relaxing2–4activities. In view of its potent effects on salt metabolism in the kidney and on vascular smooth muscle, ANF is considered to play an important part in the control of fluid volume and vascular function. Several different ANF peptides varying in size have been isolated3–10and their amino acid sequences determined4,6–10. Analysis of the sequences of the peptides suggests that they are derived by proteolysis from the same precursor. To examine this hypothesis, we have cloned cDNAs of the ANF precursor using rat atrial mRNA, determined its nucleotide sequence and deduced its amino acid sequence. The ANF precursor consists of 152 amino acid residues including a putative signal peptide sequence. This sequence contains the amino acid sequences of all the ANF peptides reported to date4,6–10.