Rapid prototyping of microstructures by soft lithography for biotechnology.
Rapid prototyping of microstructures by soft lithography for biotechnology.
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DOI：
10.1007/978-1-60327-106-6_3
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发表时间：
2010
影响因子：
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通讯作者：
D. Wolfe;D. Qin;G. Whitesides
中科院分区：
文献类型：
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作者：
D. Wolfe;D. Qin;G. Whitesides
本章介绍了利用软光刻制造微结构的方法和具体步骤。这些技术对于生物技术应用中有用的设备的原型是有用的。软光刻制造不需要专门或昂贵的设备；所需的材料和设施通常在学术界和工业界的生物和化学实验室中找到。材料成本低，从设计到原型设备的时间可以很短(24小时)，这两个事实相结合，使得使用和快速筛选也可以一次性使用的设备成为可能。在这里，我们描述了制造横向尺寸小至1μm的微结构的过程。这些类型的微结构对于微流控设备、基于细胞的分析和生物工程表面很有用。
This chapter describes the methods and specific procedures used to fabricate microstructures by soft lithography. These techniques are useful for the prototyping of devices useful for applications in biotechnology. Fabrication by soft lithography does not require specialized or expensive equipment; the materials and facilities necessary are found commonly in biological and chemical laboratories in both academia and industry. The combination of the fact that the materials are low-cost and that the time from design to prototype device can be short (< 24 h) makes it possible to use and to screen rapidly devices that also can be disposable. Here we describe the procedures for fabricating microstructures with lateral dimensions as small as 1 μm. These types of microstructures are useful for microfluidic devices, cell-based assays, and bioengineered surfaces.