Characterization of the role of the photosensitizer, deuteporfin, in the detection of lymphatic metastases in a pancreatic cancer xenograft model.
Characterization of the role of the photosensitizer, deuteporfin, in the detection of lymphatic metastases in a pancreatic cancer xenograft model.
复制标题
DOI：
10.3892/ol.2015.3441
复制
发表时间：
2015-09
期刊：
影响因子：
2.9
通讯作者：
Xinzhe Yu;Hengchao Li;D. Fu;C. Jin;J. Li
中科院分区：
文献类型：
--
作者：
Xinzhe Yu;Hengchao Li;D. Fu;C. Jin;J. Li
目前，使用光敏剂作为示踪剂来检测淋巴转移是胰腺癌治疗领域的一个正在发展的研究领域。在本研究中，氘代泊芬（一种新型光敏剂）被用作示踪剂来检测胰腺癌异种移植模型中的淋巴转移。在 Sprague-Dawley 大鼠中静脉内给药并将多特泊芬注射到左后足垫后，研究了多特泊芬的生物分布和药代动力学。通过应用伍德灯直接观察到癌组织和正常组织之间的氘泊芬浓度差异的增加。此外，高度淋巴BxPC-3-LN5人转移性胰腺癌细胞系是通过体内连续筛选和接种方法从BxPC-3细胞产生的。建立BxPC-3-LN5人胰腺癌细胞系裸鼠左后足垫异种移植模型。然后在胰腺癌异种移植模型中表征了多替泊芬作为示踪剂在检测淋巴转移中的作用。静脉给药后，与左后足垫给药组相比，多特泊芬在胰腺和腘窝淋巴结中迅速富集。此外，氘泊芬似乎在胰腺癌异种移植模型的癌性胰腺淋巴结中选择性富集。这些结果表明，氘泊芬可能被开发为一种新型光敏示踪剂，用于检测胰腺癌的淋巴转移。多替泊芬的优点是组织摄取高，具有选择性肿瘤靶向作用，并且可以静脉注射，因此适合手术治疗。
Currently, the use of photosensitizers as tracer agents to detect lymphatic metastases is a developing area of study in the field of pancreatic cancer treatment. In the present study, deuteporfin, a novel photosensitizer, was used as a tracer agent to detect lymphatic metastases in a pancreatic cancer xenograft model. The biodistribution and pharmacokinetics of deuteporfin, following intravenous administration and injection of deuteporfin into the left rear footpad, were investigated in Sprague-Dawley rats. The increased difference in deuteporfin concentration between the cancerous and normal tissues was directly observed through the application of a Wood's lamp. In addition, the highly lymphatic BxPC-3-LN5 human metastatic pancreatic cancer cell line was generated from BxPC-3 cells using a continuous screening and seeding method in vivo. A xenograft model of the BxPC-3-LN5 human pancreatic cancer cell line transplanted into the left rear footpad of nude mice, was established. The effects of deuteporfin as a tracer agent in the detection of lymphatic metastases were then characterized in the pancreatic cancer xenograft model. Following intravenous administration, deuteporfin was rapidly enriched in the pancreas and popliteal fossa lymph nodes compared with that of the left rear footpad administration group. In addition, deuteporfin appeared to be selectively enriched in the cancerous pancreatic lymph nodes of the pancreatic cancer xenograft model. These results indicated that deuteporfin may be developed as a novel photosensitizer tracer agent for the detection of lymphatic metastases in pancreatic cancer. The advantages of deuteporfin are that it has a selective tumor-targeting effect due to high tissue uptake, and that it may be administered intravenously and is therefore suitable for surgery.