Effect of maternal nicotine exposure on neonatal rat lung development: protective effect of maternal ascorbic acid supplementation
Effect of maternal nicotine exposure on neonatal rat lung development: protective effect of maternal ascorbic acid supplementation
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DOI：
10.3109/01902148.2010.515650
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发表时间：
2011-02-01
影响因子：
1.7
通讯作者：
Rayise, S. S.
中科院分区：
文献类型：
--
作者：
Maritz, G. S.;Rayise, S. S.
先前的研究表明，母亲在怀孕和哺乳期间暴露于尼古丁会干扰胎儿和新生儿的肺部生长发育。这表明母体尼古丁暴露对后代肺部的不良影响可能是由于这些肺部对氧化剂的保护不足。本研究采用Wistar大鼠。交配后将大鼠随机分为4组，即对照组、只接受尼古丁的组、只接受维生素C的组和同时接受尼古丁和维生素C的组。本研究的目的是：首先，确定妊娠和哺乳期母体尼古丁暴露（1 mg/kg体重/天，皮下）对子代肺部的影响；其次，测试皮下注射维生素C （0.5 mg/kg体重/天）是否影响肺部发育；最后，确定皮下注射维生素C是否会防止母体尼古丁暴露对后代肺部发育的不利影响。形态学和形态计量学技术被用来确定尼古丁和维生素C对出生后14、21和42天的后代肺部发育的影响。结果表明，母亲只暴露于尼古丁或维生素C只会导致后代肺实质的逐渐恶化。这些变化，类似于显微镜下的肺气肿，只有在后代的肺成熟后才变得明显。那些通过胎盘和母乳同时接触尼古丁和维生素C的动物受到的影响较轻。在妊娠期和哺乳期皮下注射维生素C来预防吸烟和尼古丁对发育中的肺部的影响也是不可取的，应该研究其他的策略。
In previous studies it was shown that maternal nicotine exposure during pregnancy and lactation interfered with fetal and neonatal lung growth and development. It was suggested that the adverse effects of maternal nicotine exposure on the lungs of the offspring may be due to inadequate protection of these lungs against oxidants. Wistar rats were used in this study. After mating the rats were randomly assigned into 4 groups, namely a control group, a group receiving only nicotine, a group exposed to only vitamin C, and a group exposed to both nicotine and vitamin C. The aim of this study was, firstly, to determine the effect of maternal nicotine exposure (1 mg/kg body weight/day, subcutaneously) during gestation and lactation on the lungs of the offspring; secondly, to test whether the subcutaneous administration of vitamin C (0.5 mg/kg body weight/day) influences lung development; and, lastly, to determine whether subcutaneous administration of vitamin C will prevent the adverse effects of maternal nicotine exposure on lung development in the offspring. Morphologic and morphometric techniques were used to determine the effect of nicotine and vitamin C on lung development in the offspring on postnatal days 14, 21, and 42. The results showed that maternal exposure to nicotine only or vitamin C only resulted in a gradual deterioration of the parenchyma of the lungs of the offspring. These changes, which resembled microscopic emphysema, only became evident after the lungs of the offspring reached maturation. Those animals that were exposed to both nicotine and vitamin C via the placenta and mother's milk were less severely affected. It is also not advisable to use subcutaneous administration of vitamin C during gestation and lactation to prevent smoke-and nicotine-related effects on the developing lung, and other strategies should be investigated.