ANGIOTENSINOGEN GENE IS EXPRESSED AND DIFFERENTIALLY REGULATED IN MULTIPLE TISSUES OF THE RAT
ANGIOTENSINOGEN GENE IS EXPRESSED AND DIFFERENTIALLY REGULATED IN MULTIPLE TISSUES OF THE RAT
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DOI：
10.1172/jci112566
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发表时间：
1986-07-01
影响因子：
15.9
通讯作者：
HABENER, JF
中科院分区：
文献类型：
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作者：
CAMPBELL, DJ;HABENER, JF
为了确定局部合成血管紧张素原在组织血管紧张素生成中的作用，我们对四组大鼠的17个不同组织中的血管紧张素原信使RNA(MRNA)水平进行了定量测定：对照组、去肾大鼠、地塞米松、乙炔雌二醇和三碘甲腺原氨酸治疗组大鼠，以及地塞米松、乙炔雌二醇和三碘甲腺原氨酸治疗肾切除大鼠。血管紧张素原基因在肝、肾、脑、脊髓、主动脉、肠系膜、心房、肺、肾上腺、大肠、胃和脾等12个组织中均有表达。血管紧张素原基因在脑垂体、脑室、睾丸、小肠和胰腺中均未检测到。当表达每克组织湿重时，肝外组织的血管紧张素原mRNA水平是肝脏水平的4%。然而，当每毫克总RNA表达时，脑、脊髓、主动脉和肠系膜的血管紧张素原mRNA水平是肝脏水平的26-42%。血管紧张素原mRNA水平的调节是组织特异性的。血管紧张素原mRNA在组织中的广泛分布可能表明局部血管紧张素系统也有类似的广泛分布，这种分布不依赖于循环中的肾素-血管紧张素系统。
To define the role of local synthesis of angiotensinogen in tissue angiotensin production, we have quantitated angiotensinogen messenger RNA (mRNA) levels in 17 different tissues of four groups of rats: control rats, nephrectomized rats, rats given dexamethasone, ethynylestradiol, and triiodothyronine, and nephrectomized rats given dexamethasone, ethynylestradiol, and triiodothyronine. Angiotensinogen mRNA was identified in 12 tissues: liver, kidney, brain, spinal cord, aorta, mesentery, atria, lung, adrenal, large intestine, stomach, and spleen. Angiotensinogen mRNA was not identified in pituitary, ventricle, testis, small intestine, or pancreas. When expressed per gram tissue wet weight, angiotensinogen mRNA levels of extrahepatic tissues were < 4% of hepatic levels. However, when expressed per milligram total RNA, angiotensinogen mRNA levels of brain, spinal cord, aorta, and mesentery were 26-42% of hepatic levels. Regulation of angiotensinogen mRNA levels was tissue specific. This demonstration of a widespread tissue distribution of angiotensinogen mRNA may indicate a similarly widespread distribution of local angiotensin systems that are independent of the circulating renin-angiotensin system.