Fast and Quantitative NMR Metabolite Analysis Afforded by a Paramagnetic Co-Solute
Fast and Quantitative NMR Metabolite Analysis Afforded by a Paramagnetic Co-Solute
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DOI：
10.1002/anie.201908006
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发表时间：
2019-09-11
影响因子：
16.6
通讯作者：
Luchinat, Claudio
中科院分区：
文献类型：
--
作者：
Mulder, Frans A. A.;Tenori, Leonardo;Luchinat, Claudio
NMR光谱是测定小分子化学特性和结构的不可或缺的技术。该方法因其稳健性和内在的定量性质而特别受到认可，并已成为从化学合成到代谢组学等不同应用领域的关键分析平台。不幸的是，自旋晶格（T-1）弛豫的核自旋极化的缓慢恢复导致大多数实验时间浪费在空闲等待上。此外，真正的定量NMR（qNMR）光谱法需要等待时间是样品中最长T-1的5倍，使得qNMR光谱法缓慢且效率低下。我们在这里证明，共溶质顺磁弛豫可以同时缓解这两个问题。加入少量的顺磁性钆螯合物，可在商业造影剂溶液的形式，使廉价，定量，高效的高通量混合物分析。
NMR spectroscopy is an indispensable technique for the determination of the chemical identity and structure of small molecules. The method is especially recognized for its robustness and intrinsically quantitative nature, and has manifested itself as a key analytical platform for diverse fields of application, ranging from chemical synthesis to metabolomics. Unfortunately, the slow recovery of nuclear spin polarization by spin-lattice (T-1) relaxation causes most experimental time to be lost on idle waiting. Furthermore, truly quantitative NMR (qNMR) spectroscopy requires waiting times of 5-times the longest T-1 in the sample, making qNMR spectroscopy slow and inefficient. We demonstrate here that co-solute paramagnetic relaxation can mitigate these two problems simultaneously. The addition of a small amount of paramagnetic gadolinium chelate, available in the form of commercial contrast-agent solutions, enables cheap, quantitative, and efficient high-throughput mixture analysis.