Highly sensitive SERS analysis of the cyclic Arg-Gly-Asp peptide ligands of cells using nanogap antennas
Highly sensitive SERS analysis of the cyclic Arg-Gly-Asp peptide ligands of cells using nanogap antennas
复制标题
使用纳米间隙天线对细胞的环状 Arg-Gly-Asp 肽配体进行高灵敏度 SERS 分析
DOI：
10.1002/jbio.201500327
复制
发表时间：
2016
影响因子：
2.8
通讯作者：
and Masahiko Hara
中科院分区：
文献类型：
--
作者：
Alejandro Portela;Taka-aki Yano;Christian Santschi;Olivier J. F. Martin;Hitoshi Tabata;and Masahiko Hara
细胞的环状RGD （cRGD）肽配体已广泛应用于多种癌症的治疗。我们报道了一种在水环境中使用单二聚体纳米间隙天线的表面增强拉曼光谱（SERS）对c（RGDfC）的高灵敏度分析。SERS特征峰与本体c（RGDfC）的拉曼光谱特征峰与其肽组分之间具有良好的一致性。SERS光谱的闪烁和强度波动的同步，表明单二聚体纳米间隙天线获得的SERS光谱以单分子到少数分子的光谱为主。c（RGDfC）的SERS光谱可以在纳米尺度上检测细胞跨膜蛋白与其配体的结合。循环RGD在纳米间隙天线上的SERS。
The cyclic RGD (cRGD) peptide ligands of cells have become widely used for treating several cancers. We report a highly sensitive analysis of c(RGDfC) using surface enhanced Raman spectroscopy (SERS) using single dimer nanogap antennas in aqueous environment. Good agreement between characteristic peaks of the SERS and the Raman spectra of bulk c(RGDfC) with its peptide's constituents were observed. The exhibited blinking of the SERS spectra and synchronization of intensity fluctuations, suggest that the SERS spectra acquired from single dimer nanogap antennas was dominated by the spectrum of single to a few molecules. SERS spectra of c(RGDfC) could be used to detect at the nanoscale, the cells' transmembrane proteins binding to its ligand.SERS of cyclic RGD on nanogap antenna.