STRUCTURAL MODIFICATIONS ASSOCIATED WITH SYNAPTIC DEVELOPMENT IN AREA CA1 OF RAT HIPPOCAMPAL ORGANOTYPIC CULTURES
STRUCTURAL MODIFICATIONS ASSOCIATED WITH SYNAPTIC DEVELOPMENT IN AREA CA1 OF RAT HIPPOCAMPAL ORGANOTYPIC CULTURES
复制标题
DOI：
10.1016/0165-3806(93)90108-m
复制
发表时间：
1993-01-15
期刊：
影响因子：
--
通讯作者：
MULLER, D
中科院分区：
文献类型：
--
作者：
BUCHS, PA;STOPPINI, L;MULLER, D
使用形态学技术，我们描述了在培养基和富含 CO2 的大气之间的界面处维持的器官型海马培养物 CA1 区移植后第一周内发生的发育重组。锥体神经元从垂直重新分布到三维培养物中间的水平细胞层，顶端树突在锥体层上方运行。胶质细胞重新分布在培养物底部的薄层中，形成组织和培养基之间的界面。星形胶质细胞被确定为数量最多的非神经元细胞。除了在移植后第一天短暂增加之外，没有观察到神经胶质细胞增殖的迹象。放射层突触接触密度在移植后立即下降，培养4周后增加约20倍，达到顶层近端部分的值，仅略小于1月龄大鼠的测量值。接受海马体外部连接的树突层最远端部分的突触密度仍然显着低于体内。脊柱与轴接触的比例与体内发现的比例相当。这些结果表明，器官型培养物的界面类型可以用作体外突触发育研究的有趣模型。
Using morphological techniques, we characterized the developmental reorganization that takes place during the first weeks after explantation in area CA1 of organotypic hippocampal cultures maintained at the interface between medium and a CO2-enriched atmosphere. Pyramidal neurones redistributed from a vertical into an horizontal cell layer in the middle of a three-dimensional culture, with apical dendrites running above the pyramidal layer. Glial cells redistributed into a thin layer at the bottom of the culture, forming an interface between tissue and culture medium. Astrocytes were identified as the most numerous non neuronal cells. No sign of glial proliferation could be observed, except for a transient increase during the first days after explantation. The density of synaptic contacts in the stratum radiatum decreased immediately after explantation and then increased by about 20-fold to reach values in the proximal part of the apical layer after 4 weeks in culture which were only slightly smaller than those measured in 1-month-old rats. The synaptic density in the most distal part of the dendritic layer which receives connections extrinsic to the hippocampus remained significantly lower than in vivo. The ratio of spine to shaft contacts was comparable to that found in vivo. These results indicate that interface type of organotypic cultures can be used as an interesting model for studies of synaptic development in vitro.