Nocardioides agrisoli sp. nov., isolated from farmland soil.
Nocardioides agrisoli sp. nov., isolated from farmland soil.
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DOI：
10.1099/ijsem.0.002158
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发表时间：
2017-09
影响因子：
2.8
通讯作者：
Xiang Wang;Wankui Jiang;Meng-Di Cui;Zhangong Yang;Xing Yu;Gang Hu;Hao Zhang;Q. Hong
中科院分区：
文献类型：
--
作者：
Xiang Wang;Wankui Jiang;Meng-Di Cui;Zhangong Yang;Xing Yu;Gang Hu;Hao Zhang;Q. Hong
从江苏省南京市农田土壤中分离到一株革兰氏染色阳性细菌djl-8 T。菌株djl-8 T的细胞为好氧、不运动、不形成孢子的棒状细胞。微生物在25-37 °C、pH 5.5-8.0和0.5- 4.0%NaCl（w/v）下生长。DNA G+C含量为69.3mol%。细胞壁肽聚糖中的诊断性二氨基酸是LL-2，6-二氨基庚二酸。主要脂肪酸（> 5%）为异-C16：0、反异-C17：0、异-C15：0、10-Me C17：0和C17：1ω 8 c。呼吸醌为MK-8（H4），主要极性脂质为磷脂酰甘油、磷脂酰肌醇、二磷脂酰甘油和未知磷脂。基于16 S rRNA基因序列的系统发育分析表明，菌株djl-8 T属于拟诺卡氏菌属，与银杏拟诺卡氏菌SYP-A7303 T的相似性最高（97.1%），其次是土壤拟诺卡氏菌mbc-2 T（96.9%）、解吡啶拟诺卡氏菌OS 4 T（96.6%）和马拉东拟诺卡氏菌RP-B30 T（96.6%）。菌株djl-8 T与银杏类诺卡氏菌SYP-A7303 T的DNA-DNA相关性较低（26.9± 2.1%）。根据形态学、生理生化和化学分类学特征，将djl-8 T菌株命名为土壤拟诺卡氏菌（Nocardioides agrisoli sp. nov.）。模式菌株为djl-8 T（=KCTC 39844 T =CCTCC AB 2017058 T）。
A novel Gram-stain-positive bacterium, designated djl-8T, was isolated from farmland soil in Nanjing, Jiangsu province, PR China. Cells of strain djl-8T were aerobic, non-motile, non-spore-forming and rod-shaped. The organism grew at 25-37 °C, pH 5.5-8.0 and 0.5-4.0 % NaCl (w/v). The DNA G+C content was 69.3 mol%. The diagnostic diamino acid in the cell-wall peptidoglycan was LL-2, 6-diaminopimelic acid. The major fatty acids (>5 %) were iso-C16 : 0, anteiso-C17 : 0, iso-C15 : 0, 10-Me C17 : 0 and C17 : 1ω8c. The respiratory quinone was MK-8 (H4) and the major polar lipids were phosphatidylglycerol, phosphatidylinositol, diphosphatidylglycerol and unknown phospholipids. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain djl-8T is a member of the genus Nocardioides and shared the highest similarity with Nocardioides ginkgobilobae SYP-A7303T (97.1 %), followed by Nocardioides soli mbc-2T (96.9 %), Nocardioide spyridinolyticus OS4T (96.6 %) and Nocardioides maradonensis RP-B30T (96.6 %). Strain djl-8T exhibited low DNA-DNA relatedness with Nocardioides ginkgobilobae SYP-A7303T (26.9±2.1 %). On the basis of the morphological, physiological, biochemical and chemotaxonomic characteristics presented in this study, strain djl-8T represents a novel species of the genus Nocardioides, for which the name Nocardioides agrisoli sp. nov. is proposed. The type strain is djl-8T (=KCTC 39844T=CCTCC AB 2017058T).