Characterization of a T4+/Leu-8+ T cell clone that directly helps B cell Ig production by secreting B cell differentiation factor.
Characterization of a T4+/Leu-8+ T cell clone that directly helps B cell Ig production by secreting B cell differentiation factor.
复制标题
通过分泌 B 细胞分化因子直接帮助 B 细胞 Ig 生产的 T4 /Leu-8 T 细胞克隆的表征。
DOI：
10.4049/jimmunol.135.1.339
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发表时间：
1985
影响因子：
4.4
通讯作者：
A. Fauci
中科院分区：
文献类型：
--
作者：
H. Goldstein;D. Volkman;J. Ambrus;A. Fauci
通过植物血凝素激活和白细胞介素 2 (IL 2) 增殖建立人 T4+/Leu-8+ T 细胞克隆 (YA2)。该克隆的功能表征表明，它为在少量自体 T 细胞存在下商陆有丝分裂原刺激的 B 细胞或在 B 细胞生长因子 (BCGF) 存在下金黄色葡萄球菌 Cowan I (SAC) 激活的 B 细胞产生 Ig 提供了有力的帮助。 YA2 对静息 B 细胞没有帮助，对与 BCGF 或 SAC 激活的 B 细胞一起培养的未激活 B 细胞有最小的帮助。通过IL 2 刺激从克隆YA2产生的上清液具有显着的B细胞分化活性，但没有BCGF或IL 2 活性。因此，YA2 仅对 B 细胞而言是 T4+/Leu-8+ 的有效直接辅助细胞，由于其选择性分泌分化因子，而不是激活和生长因子，因此 B 细胞被激活和增殖。表型定义的 T 细胞克隆群体的可用性，其具有与所选 B 细胞嗜性因子的分泌相关的有限功能辅助活性，应该有助于剖析个体 T 细胞亚群在人类 B 细胞周期调节中的作用。
A human T4+/Leu-8+ T cell clone (YA2) was established by phytohemagglutinin activation and interleukin 2 (IL 2) propagation. Functional characterization of this clone demonstrated that it provided potent help towards Ig production by pokeweed mitogen-stimulated B cells in the presence of small numbers of autologous T cells or by Staphylococcus aureus Cowan I (SAC)-activated B cells in the presence of B cell growth factor (BCGF). YA2 provided no help to resting B cells and minimal help to either unactivated B cells cultured with BCGF or SAC-activated B cells. Supernatant generated from clone YA2 by IL 2 stimulation had significant B cell differentiation activity but no BCGF or IL 2 activity. Thus, YA2 is a T4+/Leu-8+ potent direct helper only to B cells that are activated and proliferating due to its selective secretion of a differentiation factor, and not an activation and growth factor. The availability of phenotypically defined cloned populations of T cells with restricted functional helper activity related to the secretion of selected B cell tropic factors should prove useful in the dissection of the role of individual T cell subsets in the regulation of the human B cell cycle.