Molecular cloning and transcript analysis of fem-3, a sex-determination gene in Caenorhabditis elegans.
Molecular cloning and transcript analysis of fem-3, a sex-determination gene in Caenorhabditis elegans.
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秀丽隐杆线虫性别决定基因 fem-3 的分子克隆和转录分析。
DOI：
10.1101/gad.2.5.606
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发表时间：
1988
影响因子：
10.5
通讯作者：
Kimble,J
中科院分区：
文献类型：
--
作者：
Rosenquist,TA;Kimble,J
在线虫中，Fem-3基因是决定雄性命运的必需基因：XO雄性需要Fem-3在胞体和生殖系中进行雄性分化；XX两性需要Fem-3来产生精子。我们利用转座子标签技术克隆了Fem-3基因。在转座元件Tc1可移动的菌株中产生的8个自发的fem-3突变中，有6个在基因组的2kb区域包含Tc1插入。在这个区域，我们已经鉴定了三个Fem-3转录本。胚胎中存在1.7kb和1.62kb两个基因，L4s和成体中存在1.62kb和1.55kb两个基因。Fem-3转录本不是XO特有的；然而，在XX成年两性人中，它们似乎仅限于生殖系--一种与男性发育有关的组织(包括精子发生和Fem-3对胚胎的母体贡献)。XO胚胎中Fem-3RNA的含量大约是XX胚胎的6倍。讨论了这种差异在XO胚胎中指定雄性发育而不是在XX胚胎中指定男性发育的意义。
The fem-3 gene is required for specification of the male fate in the nematode Caenorhabditis elegans: XO males need fem-3 for male differentiation in both soma and germ line; XX hermaphrodites need it for the production of sperm. We have cloned fem-3 by transposon tagging. Among eight spontaneous fem-3 mutations generated in a strain in which the transposable element Tc1 is mobile, six contain Tc1 insertions in a 2-kb region of the genome. From this region, we have identified three fem-3 transcripts. Two, 1.7 kb and 1.62 kb, are presented in embryos, and two, 1.62 kb and 1.55 kb, are present in L4s and adults. The fem-3 transcripts are not XO specific; however, in XX adult hermaphrodites, they appear to be limited to the germ line--a tissue involved in male development (both for spermatogenesis and for the maternal contribution of fem-3 to the embryo). The amount of fem-3 RNA in XO embryos is approximately sixfold greater than in XX embryos. The significance of this difference in specifying male development in XO but not in XX embryos is discussed.