Carcinoembryonic antigen detection with "Handing"-controlled fluorescence spectroscopy using a color matrix for point-of-care applications
Carcinoembryonic antigen detection with "Handing"-controlled fluorescence spectroscopy using a color matrix for point-of-care applications
复制标题
使用颜色矩阵通过“Handing”控制的荧光光谱进行癌胚抗原检测，用于护理点应用
DOI：
10.1016/j.bios.2016.10.052
复制
发表时间：
2017-04-15
影响因子：
12.6
通讯作者：
Cui, Daxiang
中科院分区：
文献类型：
--
作者：
Qin, Weijian;Wang, Kan;Cui, Daxiang
在本研究中，我们基于嵌入式技术开发了一种名为Handing的无电源、准确、便携式诊断平台，可以快速检测横流试纸（LFTS）上量子点（QD）的荧光信号。 Handing 系统由三个组件组成：手持式测试终端、LFTS 盒和数据服务器。手持式测试终端是主要的系统组件，它使用印刷电路板封装与微小组件集成，通过特定程序进行图像采集、处理和数据处理。采用黑色光滑外壳和三维打印测试盒，方便检测终端的便携性，提供封闭的检测过程，增强抗干扰能力。数据服务器的功能包括预编辑、存储、范围查询、国际网络共享等。多个手持终端设备可以同时链接到同一个数据服务器。在本研究中，我们使用 QD-LFTS 系统检测肿瘤标志物癌胚抗原 (CEA)，该系统可以在 1-100 ng/mL 范围内进行定量分析，理想检测限为 0.049 ng/mL。因此，该系统适合检测临床可接受范围内的CEA。我们还使用 Handing 系统检测了 70 份阳性和 30 份阴性血清样本，表现出良好的特异性和敏感性。因此，汉鼎具有高稳定性和重复性的快速定量检测能力，因此可用于实验室和其他条件下的体外诊断，用于各种应用，例如食品评价、疾病筛查、环境监测和药物检测。
In this study, we developed a power-free, accurate, and portable diagnostic platform called Handing based on embedded technology, which can rapidly detect fluorescent signals from quantum dots (QDs) on lateral flow test strips (LFTSs). The Handing system has three components: a hand-held test terminal, LFTS cartridge, and data server. The hand-held test terminal is the primary system component and it is integrated with tiny components using printed circuit board packaging for image acquisition, processing, and data handling by a specific program. A black smooth shell and three-dimensional printed test cartridge are used to facilitate the portability of the detection terminal, which provides a closed detection process as well as enhancing the anti-interference capacity. The functions of the data server comprise pre-editing, storage, range querying, and sharing with an international network. Multiple hand-held terminal devices can be linked simultaneously to the same data server. In this study, we detected the tumor marker carcinoembryonic antigen (CEA) using the QD-LFTS system, which allowed quantitative analysis in the range of 1-100 ng/mL with an ideal detection limit of 0.049 ng/mL. Thus, the system is suitable for detecting CEA in the clinically accepted range. We also detected 70 positive and 30 negative serum samples using the Handing system, which exhibited good specificity and sensitivity. Thus, Handing has the capacity for rapid quantitative detection with high stability and repeatability, so it could be used for in vitro diagnostics in the laboratory and in other conditions for various applications, e.g., food evaluation, disease screening, environmental monitoring, and drug testing.