Deep tissue multiphoton microscopy using longer wavelength excitation
Deep tissue multiphoton microscopy using longer wavelength excitation
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DOI：
10.1364/oe.17.013354
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发表时间：
2009-08-03
期刊：
影响因子：
3.8
通讯作者：
Xu, Chris
中科院分区：
文献类型：
--
作者：
Kobat, Demirhan;Durst, Michael E.;Xu, Chris
我们通过对小鼠大脑中荧光标记血管的活体和离体双光子荧光显微镜（TPM）成像，比较了775纳米激发所达到的最大双光子荧光显微镜成像深度与1280纳米激发所达到的深度。我们使用1280纳米激发时，在大约两倍于775纳米激发的深度处实现了血管的高对比度成像。在1280纳米波长下，对成年小鼠大脑进行活体成像时，在样品表面约1纳焦的脉冲能量下，可达到1毫米的成像深度。我们还在900微米的深度进行了血流速度测量。（C）2009美国光学学会
We compare the maximal two-photon fluorescence microscopy (TPM) imaging depth achieved with 775-nm excitation to that achieved with 1280-nm excitation through in vivo and ex vivo TPM of fluorescently-labeled blood vessels in mouse brain. We achieved high contrast imaging of blood vessels at approximately twice the depth with 1280-nm excitation as with 775-nm excitation. An imaging depth of 1 mm can be achieved in in vivo imaging of adult mouse brains at 1280 nm with approximately 1-nJ pulse energy at the sample surface. Blood flow speed measurements at a depth of 900 mu m are performed. (C) 2009 Optical Society of America