Induction of myogenin messenger ribonucleic acid in rat skeletal muscle after 1 hour of passive repetitive stretching
Induction of myogenin messenger ribonucleic acid in rat skeletal muscle after 1 hour of passive repetitive stretching
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DOI：
10.1016/s0003-9993(03)00406-4
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发表时间：
2004-01-01
影响因子：
4.3
通讯作者：
Tanaka, N
中科院分区：
文献类型：
--
作者：
Ikeda, S;Yoshida, A;Tanaka, N
李文，李文.被动重复拉伸1小时后大鼠骨骼肌肌细胞生成素信使核糖核酸的诱导。Arch Phys Med Rehabil 2004;85：166- 7.目的：研究活体大鼠肌肉重复被动牵拉对肌细胞生成素信使核糖核酸（mRNA）表达的影响。设计：病例对照研究。设置：大学实验室。动物：17只雌性8周龄Wistar大鼠。干预措施：重复拉伸在麻醉大鼠的腓肠肌上手动操作（15次/min）15、30和60分钟。结果：反复牵拉麻醉大鼠腓肠肌1h后，肌细胞生成素mRNA的表达增加21.5%，肌细胞生成素mRNA的表达增加21.5%。大鼠骨骼肌反复被动牵张60分钟可诱导成肌转录因子myogenin mRNA的表达。这些发现表明被动重复拉伸在无意识或瘫痪患者中保持肌肉力量的临床实用性。
Ikeda S, Yoshida A, Matayoshi S, Horinouchi K, Tanaka N. Induction of myogenin messenger ribonucleic acid in rat skeletal muscle after 1 hour of passive repetitive stretching. Arch Phys Med Rehabil 2004;85:166-7.Objective: To investigate the effect of repetitive passive stretch of living rat muscle on myogenin messenger ribonucleic acid (mRNA) expression.Design: Case-controlled study.Setting: University laboratory.Animals: Seventeen female 8-week-old Wistar rats.Interventions: Repetitive stretch (15 times/min) was performed manually on gastrocnemius muscle of anesthetized rats for 15, 30, and 60 minutes.Main Outcome Measures: Total RNA was extracted, after the animals were killed, from the gastrocnemius muscle, and Northern blotting was performed using oligonucleotide complementary to myogenin.Results: Repetitive stretch to gastrocnemius muscles of anesthetized rats for 1 hour induced a 21.5% increase in the expression of myogenin mRNA.Conclusions: Repetitive passive stretch of the rat skeletal muscle for 60 minutes induced the expression of myogenic transcription factor myogenin mRNA. These findings suggest the clinical utility of passive repetitive stretch to maintain muscle strength in patients who are unconscious or paralyzed.