A highly sensitive acidic pH fluorescent probe and its application to HepG2 cells.
A highly sensitive acidic pH fluorescent probe and its application to HepG2 cells.
复制标题
DOI：
10.1039/b807581f
复制
发表时间：
2009-02
期刊：
影响因子：
--
通讯作者：
Wenshen Zhang;B. Tang;Xia Liu;Yuanyuan Liu;Kehua Xu;Jianping Ma;Li-li Tong;Guiwen Yang
中科院分区：
文献类型：
--
作者：
Wenshen Zhang;B. Tang;Xia Liu;Yuanyuan Liu;Kehua Xu;Jianping Ma;Li-li Tong;Guiwen Yang
一种新型酸性荧光探针 1 已被设计、合成、表征并在体内评估，作为细胞内 H(+) 的光学成像。探针的设计策略基于罗丹明染料的螺环（非荧光）和开环（荧光）形式之间的结构变化。该探针具有高灵敏度、良好的光稳定性、优异的细胞膜通透性和较强的pH依赖性。 pH滴定表明，在pH 4.2-6.0范围内，荧光强度增加100倍以上，pK(a)值为4.85，这对于研究活细胞中的酸性细胞器具有重要价值。 HepG2细胞的荧光成像也表明所设计的探针在监测活细胞内的细胞内H(+)方面具有巨大的价值。
A novel acidic fluorescent probe 1 has been designed, synthesized, characterized and evaluated in vivo as optical imaging of intracellular H(+). The design strategy for the probe is based on the change in structure between spirocyclic (non-fluorescent) and ring-open (fluorescent) forms of rhodamine dyes. The probe exhibits high sensitivity, good photostability, excellent cell membrane permeability and strong pH dependence. The pH titration indicates that the fluorescence intensity increases more than 100-fold within the pH range of 4.2-6.0 with the pK(a) value of 4.85, which is valuable for studying acidic organelles in living cells. The fluorescent imaging of HepG2 cells also demonstrates that the designed probe has great value in monitoring intracellular H(+) within living cells.