EFFECT OF MOLECULAR-SIZE, CONCENTRATION IN NUTRIENT SOLUTION, AND EXPOSURE TIME ON AMOUNT AND DISTRIBUTION OF POLYETHYLENE-GLYCOL IN PEPPER PLANTS
EFFECT OF MOLECULAR-SIZE, CONCENTRATION IN NUTRIENT SOLUTION, AND EXPOSURE TIME ON AMOUNT AND DISTRIBUTION OF POLYETHYLENE-GLYCOL IN PEPPER PLANTS
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DOI：
10.1104/pp.54.3.226
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发表时间：
1974-01-01
期刊：
影响因子：
7.4
通讯作者：
JANES, BE
中科院分区：
文献类型：
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作者：
JANES, BE
辣椒属植物辣椒用分子量为400、600、1000、1540或4000的聚乙二醇作渗透剂，在渗透势为−3.0或−5.0 bar的营养液中培养加利福尼亚奇迹。结果表明，相对分子质量为1000或1540的聚乙二醇是降低营养液水势的最佳渗透剂，植物体内聚乙二醇的少量积累与水分释放量无关。叶片汁液中聚乙二醇的浓度和总积累量与聚乙二醇的相对分子质量呈负相关，在较低渗透势的营养液中聚乙二醇浓度较大，且随时间的延长而增加。除了生长在聚乙二醇4000中的植株外，叶中的聚乙二醇量比根中的多。有迹象表明，当聚乙二醇的浓度达到每毫升1到2毫克时，任何额外吸收的量都会转移到树叶中。葡聚糖凝胶层析的结果表明，聚乙二醇通过植株并没有改变其平均分子质量。这表明，可能作为污染物存在于商业产品中的小分子没有选择性吸收。
Pepper plantsCapsicum annuum L.var. California Wonder were grown in nutrient solutions of either −3.0 or −5.0 bars osmotic potential, using polyethylene glycol with molecular weights of 400, 600, 1000, 1540, or 4000 as osmotica. Polyethylene glycol with molecular weights of 1000 or 1540 proved most satisfactory as osmotica to decrease the water potential of nutrient solutions.There was no relationship between the small amount of polyethylene glycol accumulated in the plants and the amount of water transpired. The concentration of polyethylene glycol in the expressed sap of the leaves and the total accumulated was inversely related to molecular weight of polyethylene glycol, was greater at lower osmotic potential of nutrient solution, and increased with time in solution. Except for plants grown in polyethylene glycol 4000, there was more polyethylene glycol in leaves than roots. The indications were that, when the concentration of polyethylene glycol reached a value of 1 to 2 mg per ml, any additional quantity absorbed was transferred to the leaves. The major proportion of polyethylene glycol 4000 absorbed was retained in the roots.The results of Sephadex gel chromatographs showed that the passage of polyethylene glycol through the plants did not alter the average molecular weight. This indicated that there was no selective absorption of small molecules that might be present as contaminates in the commercial product.