Tools and methods for circular dichroism spectroscopy of proteins: a tutorial review.
Tools and methods for circular dichroism spectroscopy of proteins: a tutorial review.
复制标题
DOI：
10.1039/d0cs00558d
复制
发表时间：
2021-08-07
影响因子：
46.2
通讯作者：
Wallace BA
中科院分区：
文献类型：
--
作者：
Miles AJ ;Janes RW ;Wallace BA
圆二色光谱是生物化学、结构生物学和药物化学中广泛使用的方法。在过去十年中发表的24000多篇论文包括蛋白质的CD表征;其中许多研究还包括其他补充化学，生物物理和计算化学方法。本教程回顾介绍了背景技术的CD光谱和良好的实践方法，高质量的数据收集。它特别侧重于现有的和新的方法和工具，可用于实验设计和解释，数据处理，可视化，分析，验证，存档和加入，包括开发工具，以增强该方法与其他结构和化学生物学研究的互补性。本教程综述讨论了目前的方法和资源，可用于良好的实践数据收集和圆二色光谱分析，广泛使用的方法，用于检查蛋白质的结构和构象变化。
Circular dichroism (CD) spectroscopy is a widely-used method in biochemistry, structural biology and pharmaceutical chemistry. More than 24 000 papers published in the past decade have included CD characterisations of proteins; many of those studies have also included other complementary chemical, biophysical, and computational chemistry methods. This tutorial review describes the background to the technique of CD spectroscopy and good practice methods for high quality data collection. It specifically focuses on both established and new methods and tools available for experimental design and interpretation, data processing, visualisation, analysis, validation, archiving, and accession, including tools developed to enhance the complementarity of this method with other structural and chemical biology studies. This tutorial review discusses current methods and resources available for good practice data collection and analyses of circular dichroism spectroscopy, a widely-used method for examining the structures and conformational changes of proteins.